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METHOD FOR ASSAYING CELLULASE ACTIVITY, SCREENING METHOD USING ASSAYING METHOD, AND HIGH-PERFORMANCE CELLULASE-PRODUCING BACTERIA SELECTED USING SCREENING METHOD
METHOD FOR ASSAYING CELLULASE ACTIVITY, SCREENING METHOD USING ASSAYING METHOD, AND HIGH-PERFORMANCE CELLULASE-PRODUCING BACTERIA SELECTED USING SCREENING METHOD
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机译:检测纤维素酶活性的方法,使用该检测方法的筛选方法以及使用该筛选方法选择的高性能纤维素酶生产菌
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摘要
A method for assaying cellulase activity, including: a process (A) of preparing two or more substrate solutions, which have an identical absorbance measured at an identical wavelength and in which cellulose is dispersed at an identical concentration, and measuring the absorbance of each of the substrate solutions; a process (B) of respectively adding different kinds of enzyme solutions to the substrate solutions, and performing an enzyme reaction under the same conditions; a process (C) of measuring the absorbance of each of the substrate solutions after the enzyme reaction; a process (D) of calculating the absorbance decrease values of the substrate solutions before and after the enzyme reaction; and a process (E) of assaying cellulase activities of the enzyme solutions based on the absorbance decrease values. In the assay of the process (E), it is determined that the enzyme solutions have higher cellulase activity as the absorbance decrease values become larger.
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