Subcellulosome Preparation with High Cellulase Activity from Clostridiumthermocellum

摘要

We have prepared a much simpler cellulase preparation than that of cellulosomesfrom the extracellular broth of Clostridium thermocellum. This subcellulosome preparation from C. thermocellum was obtained by column chromatography on CM-Bio-Gel A and then on the lectin-affinity material (Jacalin). The subcellulosome preparation is a macromolecular complex, composed of six main protein subunits (molecular weight 210,000 to 58,000) revealed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The specific activities of