缺氧诱导肿瘤细胞BCL-2表达促进LPPCN样细胞核凝集的形成

摘要

Objective To explore and analysis the BCL-2 hypoxia inducing tumor cells promotes the formation of LPPCN sample nucleus aggregation effect.Methods Choose B16 melanoma cells as the research object, the use of CoCL2 model to simulate hypoxia microenvironment hypoxia, using PCR and Western for the BCL-2 protein and RNA expression, positioning using immunofluorescence technique to observe the BCL-2 expression.Results Under the condition of normal oxygen, over time, and decreased the BCL-2 expression, anoxic condition, the BCL-2 expression shows the tendency of increasing with longer, 12 h after hypoxia, the BCL-2 located in the cytoplasm, anoxic 36 h, under the condition of normal oxygen the BCL-2 positioning has no obvious change, hypoxia under the condition of the BCL-2 can be found in the nucleus expression.Conclusion Hypoxia conditions can promote tumor cell formation LPPCN nucleus aggregation.%目的：探索分析缺氧诱导肿瘤细胞BCL-2表达促进LPPCN样细胞核凝集的形成影响。方法：选择B16黑色素瘤细胞为研究对象，利用CoCL2缺氧模型来模拟缺氧微环境，采用RT-PCR和Western进行BCL-2蛋白和RNA的表达影响，利用免疫荧光技术观察BCL-2表达定位情况。结果：正常氧条件下，BCL-2表达随着时间推移而下降，缺氧条件下，BCL-2表达随着时间延长而呈现逐渐上升的趋势，缺氧12h后，BCL-2定位于细胞质，缺氧36h时，正常氧条件下的BCL-2定位无明显变化，缺氧条件下的BCL-2可见细胞核表达。结论：缺氧条件可促进肿瘤细胞形成LPPCN样细胞核凝集。