海燕体壁胶原蛋白提取工艺的初步研究

摘要

Acid -soluble collagen (ASC) and pepsin- solubilized collagen (PSC) were extracted from body wall of star fish Asterina pectinifera by two different methods. The optimum extraction of ASC with the yield of 7.93％were found at hydrolyzing temperature of 10 ℃, hydrolyzing time of 72 h and the concentration of 0.5 mol/L acetic acid -sodium acetate. The optimum conditions for the extraction of PSC with yield of 49.61％ were found as follows:hydrolyzing temperature of 17 ℃, hydrolyzing time of 96 h, and the pepsin dose of 4％. The UV spectra and Amino Acid Analysis revealed that ASC and PSC from body wall of the star fish were classified as type I collagen.%采用两种不同的方法从海燕Asterina pectinifera体壁中提取出酸溶性胶原蛋白(ASC)和胃蛋白酶促溶性胶原蛋白(PSC).结果表明:ASC的最佳反应条件是,温度为10 ℃,提取液种类为乙酸-乙酸钠溶液,提取液浓度为0.5 mol/L,提取时间为72 h,ASC得率为7.93%;PSC的最佳反应条件是,温度为17℃、胃蛋白酶酶加量为4%、提取时间为96 h,PSC得率为49.61%.经紫外光谱和氨基酸组成分析,结果表明,ASC和PSC均为典型的Ⅰ型胶原蛋白.