ISOLATION OF PEPSIN-SOLUBILIZED COLLAGEN (PSC) FROM CRUDE COLLAGEN EXTRACTED FROM BODY WALL OF SEA CUCUMBER (BOHADSCHIA SPP).

摘要

Introduction: Sea cucumber is a marine invertebrate. About 70% of the total body wall protein of sea cucumber is accounted for highly insoluble collagen fibers. Objectives: The aim of this study was to isolate pepsin-solubilized collagen (PSC) from crude collagen, extracted from body wall of sea cucumber Bohadschia spp. Methods: Body wall of Bohadshia spp were cut into small pieces followed by washing with distilled water and then replaced with 4 mM ethylenediaminetetraacetic acid (EDTA), 0.1 M Tris–HCl, pH 8.0, and stirred for 3 days to get precipitated crude collagen fibrils. Disaggregated Insoluble crude collagen fibrils were treated with 0.1 M NaOH and 0.5 M acetic acid containing porcine pepsin to get PSC collagen. Results: PSC was successfully isolated from disaggregated crude collagen fibres, with 65% yield. According to the electrophoretic pattern, PSC collagen was identified as type I collagen, consisting of three .1chains of approximately 138 kDa each. Conclusion: As high as 60% of PSC was successfully isolated from Bohadschia spp and classified as type I collagen. This finding shows the potential use of this collagen as an alternative to mammalian collagen used in the nutraceutical and pharmaceutical industries