TSHR和MMP-9在甲状腺乳头状癌组织中的表达及其临床意义

摘要

[目的]探讨甲状腺乳头状癌(PTC)组织中促甲状腺激素受体(TSHR)与基质金属蛋白酶(MMP -9)的表达及其与病理参数之间的关系.[方法]应用免疫组织化学(SP)法检测56例PTC,30例结节性甲状腺肿及11例正常甲状腺组织标本的TSHR和MMP -9蛋白表达.[结果]TSHR蛋白在PTC、结节性甲状腺肿和正常甲状腺组织的表达率分别为91.07％、70.00％和27.27％,三组间比较差异有显著性意义(P＜0.05)；TSHR在有淋巴结转移PTC组与无淋巴结转移组的表达率分别为76.92％与95.35 ％(P＞0.05)；在Ⅲ～Ⅳ期与Ⅰ～Ⅱ期的表达率分别为89.66％与92.60％ (P ＞0.05).MMP -9蛋白在PTC、结甲和正常甲状腺组织中表达率分别为88.37％、36.67％和0％,三组间比较差异有显著性意义(P＜0.05)；在有淋巴结转移PTC组与无淋巴结转移PTC组的表达率分别为92.31％与79.07％ (P ＞0.05),在Ⅲ～Ⅳ期与Ⅰ～Ⅱ期的表达率分别为89.66％与74.07％(P＞0.05).TSHR、MMP -9的表达与患者的性别、年龄及肿瘤大小均无相关性(P＞0.05).[结论] TSHR蛋白高表达与甲状腺乳头状癌的发生有关,可能是甲状腺癌发生的一个早期标志;MMP -9高表达可能与甲状腺癌细胞侵袭和转移密切相关.%To evaluate the expressions of thyroid stimulating hormone receptor (TSHR) and matrix metallopro-teinase ?9(MMP ?) in papillary thyroid carcinoma (PTC) and their clinical significances. [Method] The SP immunohis-tochemistry was used to detect the expressions of TSHR and MMP - 9 protein in 56 cases of PTC, 30 cases of nodular goiter and 11 cases of normal thyroid tissue. [Results] The positive expression rate of TSHR in PTC was significantly higher than those of nodular goiter and normal thyroid tissue ( P < 0. 05 ). and the rates in PTC with lymph node metastasis and without were 76.92% and 95.35% respectively(P >0.05), The rates in stage 1 - IVand stagel - Ilwere 89.66 % and 92.60 % respectively (P >0. 05) ; The positive expression rate of MMP -9 protein in PTC was significantly higher than those of nodular goiter and normal thyroid tissue (P <0.05 ) . and the rates in PTC with lymph node metastasis and without were 92.31 % and 79.07 % respectively (P >0.05) ,and the rates in stage HI - IVand stagel - Ewere 89.66 % and 74. 07 % respectively(P > 0.05 ). Their expressions of both TSHR and MMP ?9 were not related to sex, age and tumor size of the patient ( P > 0. 05 ). [ Conclusion ] Over - expression of TSHR might be related to the development of PTC and become a early marker of the thyroid carcinoma. The over 梕xpression of MMP ? could be related to invasion and metastasis.