响应面法优化莲子低聚糖超声波辅助提取工艺

摘要

Ultrasonic-assisted extraction（UAE） of oligosaccharides from lotus（Nelumbo nucifera Gaertn.） seeds（LSO） was studied in this paper.The three parameters,ultrasonic power（300～500 W）,solid to liquid ratio（g/mL） 1∶15～1∶25,and extraction time（30～50 min）,were optimized using the Box-Behnkens Design（BBD） with a quadratic regression model built by Response Surface Methodology（RSM）.The order of three parameters on the LSO yield was as follows： solid to liquid ratioultrasonic powerextraction time.The highest oil yield,1.13%（w/w）,was obtained under optimal conditions for ultrasonic power,solid to liquid ratio,and extraction time at 320 W,1∶25,and 48 min,respectively.The LSO yield extracted by UAE was significantly higher than that by using hot water extraction（HWE： 68.18%） and microwave-assisted extraction（MAE： 29.88%）.%采用响应面分析法对超声辅助提取莲子低聚糖工艺参数进行优化.研究了超声波功率（300-500 W）、料液比（g/mL）1∶15-1∶25和提取时间（30-50 min）对超声辅助提取莲子低聚糖得率的影响,对实验数据进行回归分析,优化工艺参数.结果表明：超声辅助提取各试验因素对莲子低聚糖得率的影响次序为料液比〉超声波功率〉提取时间.优化所得莲子低聚糖超声波辅助提取较佳工艺参数为：超声波功率320 W,液料比1∶25,提取时间48 min,在该条件下,低聚糖得率为1.13%.与热回流提取法和微波辅助提取法相比,超声辅助提取法使莲子低聚糖得率分别提高66.18%和29.88%.