[目的]考察可见分光光度法测定胡椒碱含量的最佳溶剂和最适宜检测波长.[方法]以二氯乙烷、乙酸乙酯和无水乙醇作为溶剂,考察胡椒碱在380～420 nm波长范围内的最佳溶剂和测定波长,并将其测定纯度与HPLC法进行比较.[结果]最佳的溶剂为无水乙醇;在较低浓度范围内(0～0.01 g/L),胡椒碱最适宜的测定波长为380 nm,而在较高浓度范围(0.20～1.00 g/L),其最适宜的测定波长则为400 nm.可见分光光度法所测定的样品中胡椒碱纯度与HPLC法进行比较,平均误差为0.85%.[结论]利用可见分光光度计测定胡椒碱纯度具有较高的准确度,是可行的.%[ Objective ] To investigate the optimal solvent and detection wavelength for piperine by UV-VIS spectrophotometry. [ Method ] Using dichloroethane, ethyl acetate and absolute ethanol as solvent, the optimal solvent and detection wavelength of piperine were surveyed at wavelength of 380 - 420 nm, and the determined result was compared with that detected by HPLC. [ Result ] The optimum solvent was absolute ethanol, and the optimum detection wavelength for piperine was 380 nm at lower concentration (0 -0.01 g/L), while that was 400 nm at higher concentration (0.20 - 1.00 g/L). The mean error between the purity determined by UV-VIS spectrophotometry and HPLC method was just 0.85％. [ Conclusion] Good in precision, UV-VIS spectrophotometry is favorable for the determination of piperine.
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