消化系统肿瘤患者外周血T淋巴细胞亚群及NK细胞分析与临床意义

摘要

Objective To observe the alteration of peripheral blood T-lymphocyte subgroups and NK cells in patients with digestive systemic cancer,and identify the condition of body s immune function of patients with digestive systemic cancer and the clinical significance of this two flow cytometric items. Methods By a 3-color flow cytometry, peripheral blood T-lymphocyte subgroups and NK cells from 123 patients with digestive systemic cancer were detected. For compares, 35 healthy persons were detected as a control group. Results In patients with digestive systemic cancer, the percentages of total T-lymphocytes (CD3+),T4 cells (CD3 + CD4+) and NK cells (CD3- CD16 + CD56 + ) clearly decreased than that of the control group(P<0. 01) ;the rates of T4/T8 (CD3 + CD4 + /CD3+CD8+) distinctly decreased than that of the control group(P<0. 05) ; moreover, the percentages of T8 cells (CD3 + CD8 ) were significantly higher tan that of the control group(P<0. 05) ;but the numerical data between various digestive systemic tumors had no significant differences(P>0. 05). Conclusion T-lymphocyte subgroups and NK cells have significant differences between the patients with digestive systemic cancer and healthy people,indicating that the cellular immune function of tumour patients is lower. It is very important to analyze the levels of peripheral blood T-lymphocyte subgroups and NK cells for evaluating patient s immune function,monitoring therapeutic effect and judging prognosis.%目的 通过观察外周血T淋巴细胞亚群及NK细胞活性在消化系统肿瘤患者中的表达,探讨消化系统肿瘤患者的细胞免疫功能状况及临床意义.方法 采用流式细胞分析法对123例消化系统肿瘤患者外周血T淋巴细胞亚群及NK细胞进行检测,并与35例健康对照组比较.结果 与健康对照组比较,消化系统各肿瘤组患者外周血总T细胞(CD3+)、T4细胞(CD3+CD4+)、NK细胞(CD3-CD16/56+ )百分率下降(P<0.01);T4/T8(CD3+CD4+/ CD3+CD8+)比值降低(P<0.05);T8细胞(CD3+CD8+)百分率明显升高(P<0.05);消化系统各肿瘤组间差异无统计学意义(P>0.05).结论 消化系统肿瘤患者外周血T淋巴细胞亚群及NK细胞活性表达较健康对照组显著降低,表明消化系统肿瘤患者细胞免疫功能低下.检测消化系统肿瘤患者外周血T淋巴细胞亚群和NK细胞水平对评估患者的细胞免疫功能、监测抗瘤疗效和判断预后均具有一定的临床意义.