目的：研究消癌平对 lewis 肺癌小鼠移植瘤生长的影响及其与细胞周期特异性化疗药联合应用抗肿瘤效果，为消癌平应用于肺癌的临床治疗提供一些新的理论依据。方法（1）预实验：①成瘤时间：复制Lewis 肺癌小鼠模型10只，于接种后第9日可触及粟粒大小结节；②细胞周期：复制 Lewis 肺癌小鼠模型30只，随机分成两组，每组15只，于种植后第10日分别腹腔注射生理盐水和消癌平注射液，分别于第12日、14日和第16日处死小鼠5只，后用流式细胞仪分析细胞周期，接种后第14日大部分瘤细胞停滞于 G1期。（2）实验：复制 Lewis 肺癌小鼠模型，数量共60只，随机分成6组，于种植后第10日开始给药，分别采用生理盐水、消癌平、氟尿嘧啶，消癌平联合氟尿嘧啶，丝裂霉素、消癌平联合丝裂霉素对其治疗，观察小鼠的生存状态，计算肿瘤生长抑制率，用免疫组化方法测定 vegf 表达水平。结果流式细胞仪分析表明消癌平将肿瘤细胞阻滞于 G1期，消癌平组、丝裂霉素组、消癌平联合丝裂霉素组3组肿瘤抑制率分别为22．6％、37．4％，42.9％，3组与对照组均有显著性差异（P ＜0．01），且消癌平联合丝裂霉素组肿瘤抑制率高于消癌平组、丝裂霉素组；生理盐水组、消癌平组、氟尿嘧啶组、消癌平联合氟尿嘧啶组各组 VEGF 表达强阳性率分别为：80.0％，57．1％，40％，20％，消癌平联合氟尿嘧啶组 VEGF 强阳性率高于其他3组。结论消癌平能明显改善小鼠生存状态，并对 Lewis 肺癌移植瘤有显著抑制作用，并能降低小鼠的死亡率，消癌平联合 G1期细胞周期特异性化疗药抑瘤效果强于单用 G1期细胞周期特异性化疗药，消癌平对 Lewis 小鼠移植瘤有显著抑制作用。其抗肿瘤作用可能与调控肿瘤细胞周期及抑制肿瘤血管生成相关。%Objective Xiaoaiping joint cell cycle specificity effective elimination effect on lewislung trans -plantation tumor growth in mice .Methods (1 )Preliminary experiments :① Time of tumor growth :Copy Lewis lung cancer model in mice ,a total of 3 0 ,on 9 days after inculation can hit millet grain size of nodules .② The cell cycle :Copy Lewis lung cancer model in mice ,again with the above method ,a total of 3 0 ,The above mice were randomly divided into two groups of 1 5 ,On the 1 0 th day intraperitoneal injection of physiological saline respec -tively and Xiaoaiping injection ,in the first twelve days ,fourteen days and sixteendays ,mice were sacrificed 5 , Cell cycle is analyzed with flow cytometry instrument ,and after 1 4 days after inoculation the stagnation of most tumor cells in G1 phase .(2 )Experiment :Copy the Lewis lung carcinoma mouse model ,a total number of 6 0 ,the mice modeling were randomly divided into six groups of 1 0 ,On for 1 0 days after inculation ,mice were treated withSaline , Xiaoaiping , Fluorouracil , XiaoaipingcombinedwithFluorouracil , Mitomycin , Xiaoaiping-combinedwithMitomycinseparately .Observe the survival state of the mouse ,and calculate the tumor growth in-hibition rate ,level of vegf expression was determined by immunohistochemical method .Results Flow cytome-try analysis showed Xiaoaiping tumor cell cycle arrest in G 1 phase ,Xiaoaiping ,Mitomycin ,Xiaoaiping combined with Mitomycin each group of tumor inhibition rate were 2 2 .6 % ,3 7 .4 % ,4 2 .9 % ,Three groups had signifi-cant difference with control group ( P < 0 .0 1 ) ,and cancer tumor inhibition rate of ,Xiaoaiping combined with Mitomycin is higher than Xiaoaiping ,Mitomycin Xiaoaiping ,Saline ,Fluorouracil ,Xiaoaiping combined with Flu-orouracil ,each group strong VEGF expression positive rate is respectively :8 0 .0 % ,5 7 .1 % ,4 0 % ,2 0 % ,elim-inate cancer flat fluorouracil with strong VEGF positive rate is higher than the normal saline group ,eliminate cancer group ,fluorouracil ,Xiaoaiping combined with Fluorouracilstrong VEGF positive rate is higher than the other three groups .Conclusion Xiaoaiping can significantly improve survival in mice ,and had a significant in-hibitory effect on Lewis lung carcinoma transplanted tumor ,and can reduce the mortality of mice ,Xiaoaiping joint G1 stage of cell cycle specificity tumor suppression effect is stronger than effective use G 1 stage of cell cycle specificity ,effective .Its antitumor effect may be related to regulation of tumor cell period and inhibit tumor an -giogenesis .
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