消癌平注射液联合紫杉醇对人卵巢癌SK-OV-3细胞增殖及裸鼠异位移植瘤生长的作用

摘要

Objective To investigate the inhibitory effect of Xiaoaiping injection (XAP) combined with paclitaxel (PTX) on human ovarian cancer SK-OV-3 cells .Methods In vitro anti-proliferation activity study of XAP combined with PTX on hu-man ovarian cancer SK-OV-3 cells was performed using optical microscope and MTT assay .Human ovarian cancer SK-OV-3 cells were treated with PTX ,XAP ,PTX combined XAP or vehicle control .Each group of cells was treated with drugs for 24 h or 48 h .SK-OV-3 cell morphology was observed with optical microscope .MTT assay was used to detect the A value and cell vi-ability was calculated .In vivo effect of XAP combined with PTX on the growth of SK-OV-3 cells was determined in nude mice . In our study ,thirty-six mice were randomly divided into six groups :G1 (NS) ,G2 (PTX ,10 mg/kg) ,G3 (XAP ,20 ml/kg) , G4 (XAP ,50 ml/kg) ,G5 (PTX 10 mg/kg+XAP 20 ml/kg) and G6 (PTX 10 mg/kg+XAP 50 ml/kg) .Animals were trea-ted for 18 days .Body weight ,tumor volume and tumor inhibition rate were recorded and calculated .The results were analyzed by the SPSS 19 .0 software .Results In vitro study showed that SK-OV-3 cell viability decreased significantly in PTX com-bined XAP group compared to PTX group or XAP group ,in a time and dose-dependent manner .In vivo study showed that the combination of PTX and XAP resulted in decreased tumor weight significantly compared to the control or the PTX alone . Conclusion The combination of XAP and paclitaxel exhibited a synergistic effect both in vitro and in vivo in nude mouse tumor xenograft model .%目的 研究消癌平(XAP)注射液和紫杉醇(PTX)联合应用对人卵巢癌SK-OV-3细胞增殖及裸鼠异位移植瘤的抑制作用.方法 将生长至对数期的人卵巢癌SK-OV-3细胞分为6组:对照组、紫杉醇组(10 nmol/L)、消癌平注射液低、高剂量组(20、80μl/ml)、消癌平注射液低、高剂量联合组(PTX 10 nmol/L+XAP 20μl/ml、PTX 10 nmol/L+XAP 80μl/ml),各组分别加入药物干预24 h或48 h后,MTT法测定并计算细胞存活率.将36只雌性荷人卵巢癌细胞S K-O V-3的异位移植瘤裸鼠随机分为6组,包括对照组(G1:生理盐水)、紫杉醇组(G2:PTX 10 mg/kg)、消癌平注射液低、高剂量组(G3:XAP 20 ml/kg、G4:XAP 50 ml/kg)、低剂量联合组(G5:PTX 10 mg/kg+XAP 20 ml/kg)以及高剂量联合组(G6:PTX 10 mg/kg+XAP 50 ml/kg),每组6只.各治疗组予相应药物治疗18 d,观察记录小鼠一般情况、体重、肿瘤体积,并计算抑瘤率.结果 SK-OV-3细胞体外实验结果显示,与对照组相比,消癌平注射液、低剂量联合组和高剂量联合组的SK-OV-3细胞存活率均显著降低(P<0.05或P<0.01),且联合用药组与紫杉醇组相比,也有显著差异(P<0.05),并呈现剂量和时间依赖性.裸鼠异位移植瘤实验结果显示,高剂量联合组的抑瘤率明显高于对照组和紫杉醇组,差异有统计学意义(P<0.05).结论 消癌平注射液和紫杉醇联用对人卵巢癌细胞SK-OV-3裸鼠异位移植瘤的抑制具有协同作用(P<0.05).