• 主题
高级搜索  >
历史搜索 清空历史
您现在的位置：首页> 中文期刊>药学>医药卫生>药学实践杂志

药学实践杂志

药学实践杂志

CSTPCD

Journal of Pharmaceutical Practice
本刊主要宗旨在于推动医院药学实践，指导临床合理用药、提高药物治疗水平；及时介绍国内外药学领域的新技术、新知识、新成就，沟通药师与医师、护师之间联系，互通情报，为临床安全、有效、经济用药提供理论和实践资料。主要以医院药师和临床医师为主要对象。
  • 发文量：3392
  • 被引量：1055
  • H指数：1
  • 开始收录时间：1983(3)
  • CNKI综合因子：0.717
  • 维普期刊影响因子： 0.452
  • 万方期刊影响因子：0.78
  • 创刊时间：1983
  • 国内刊号/CN：31-1685/R
    国际刊号/ISSN：1006-0111
  • 发行周期：双月刊
    邮发代号：4-664
  • 主管单位：第二军医大学
    主办单位：第二军医大学,中国药学会药事管理专业委员会
药学实践杂志-联系信息
  • 主编：李捷玮
  • 电话：021-81871322 81841324
  • 邮箱：yxsjzzs@163.com
  • 地址：上海市国和路325号
  • 邮编：200433
药学实践杂志-获奖情况
  • • 2018年度中国高校优秀科技期刊
  • • 2016年度中国高校编辑出版质量优秀科技期刊
  • • 第三届上海市高校优秀科技期刊奖（2018）
  • • 第二届上海市高校特色科技期刊奖（2016）
  • 收录汇总
  • 刊内文献检索
  • 投稿信息

刊期浏览

相关中文期刊

相关中文会议

相关外文期刊

相关外文会议

最新期刊

热门期刊

药学实践杂志 >2022年第4期

学科

年度

全选（0）
排序：
共4384条结果
  • 摘要：基于医学发展和临床诊疗实践的迫切需求,正电子放射性药物的研究和使用日益广泛.本文简要介绍了正电子放射性药物及其特点,回顾性分析了国内外正电子放射性药物的发展历程,针对我国正电子放射性药物的现状,探讨推进正电子放射性药物研发、生产和临床应用的设想.%Because of the urgent needs of the medical advances and clinical diagnosis practice, positron emission tomography drugs were increasingly widely researched and used. The characteristics and the course of the development of positron emission tomography drugs were reviewed at home and abroad. Based on the current status of positron emission tomography drugs in China, the ideas of promoting the research, production and clinical application of positron emission tomography drugs were also discussed in this paper.
  • 摘要：Objective To examine effect of lipoxin A4 ( LXA4), protectin Dl ( ProDl) or resolvin D1 ( RvD1 ) on the activity of NFkB and their action mechanism. Methods The CHO cells, stably expressing NFkB luciferase reporter gene, were treated with LPS, HSP70, HMGB1 or S100A4, in the presence or absence of 100 nmol/L of LXA4, ProDl or RvDl for 30 minutes. The activity of NFkB was detected with the luciferase assay. The content of tumor necrosis factor α (TNFα) in supernatant was measured by enzyme-linked immunosorbent assay (ELISA) and the expression of NFkB in the nucleus was detected by immune blotting. Results The activity of NFkB and the level of TNFα in supernatant were significantly upregulated after treatment of the cells with LPS, HSP70, HMGBI or S100A4, respectively. However, the NFkB activity and concentration of TNFα were lowered in the cells preincubated with LXA4, ProD1 or RvD1 as compared to the stimulated cells. Moreover, the lipids significantly decreased the content of NFkB in the nucleus. Conclusion LXA4, ProD1 or RvD1 could significantly inhibit the ligand-stimulated NFkB activity through interfering with NFkB Iranslocation from cytoplast to nucleus. LXA4, ProD1 and RvD1 showed the potential in the development of new anti - inflammatory therapeutics, which was required further research.%目的 探讨脂质小分子脂氧素A4 (LXA4)、保护素D1 (ProD1)、ResolvinD1( RvD1)对核因子kB (NFkB)活性的影响及作用机制.方法 稳定表达NFkB荧光素酶报告基因的中国仓鼠卵巢细胞分别由100 nmol/L LXA4、ProD1、RvD1预处理30 min后,细胞被激动剂LPS、HSP70、HMGB1或S100A4刺激.通过检测荧光素酶活性以评价脂质小分子对激动剂激活NFkB活性的作用.细胞培养上清中TNFα的含量由ELISA检测,胞核中NFkB的含量由Western印迹检测.结果 LPS、H SP70、HMGB1和S100A4显著上调NFkB的活性,增加细胞分泌的TNFα的量.LXA4、ProD1、RvD1显著抑制NFkB激活,降低细胞分泌的TNFα含量,减少NFkB的入核.结论 LXA4、ProD1、RvD1显著抑制多种激动剂活化NFkB,其作用机制可能与其能降低NFkB的入核有关,这几个脂质小分子在研制新型抗炎药物方面具有进一步开发和研究的潜力.
  • 摘要：Objective To study the relationship between steady-state plasma concentration and clinical efficacy of quetiapine in treatment of schizophrenia with the same dose. Methods 148 schizophrenia patients were treated with quetiapine with the same dose (600 mg/d). The clinical efficacy and adverse reactions were evaluated with BPRS and TESS respectively t while the plasma concentration of quetiapine was tested at 2nd, 4th and 8th weekend. Results The plasma concentration of quetiapine had significant difference with the reduced rate of BPRS (P <0.05), but had no difference with the scores of TESS. Conclusions The testing of plasma concentration could help to distinguish the effective and toxic concentration of quetiapine in treatment of schizophrenia, which could help doctors to choose rational dose of quetiapine for each patients.%目的 研究喹硫平同等剂量治疗精神分裂症时的稳态血药浓度与临床疗效间的关系.方法 以固定剂量喹硫平(600 mg/d)治疗148例精神分裂症患者,疗效和不良反应采用简明精神病评定量表(BPRS)和副反应量表(TESS)进行评定,同时测定治疗第2、4、8周的稳态血清药物浓度.结果 精神分裂症患者的BPRS减分率与血药浓度之间差异有显著性(P＜0.05),血药浓度与不良反应(TESS分值)之间差异无显著性(P＞0.05).结论 喹硫平的血药浓度检测有助于确定药物有效浓度及毒性浓度之间的范围,做到用药个体化,指导临床合理用药.
  • 摘要：Natural repellent had the characteristic of safely, environmental friendliness, little toxicity and so on. Its research was mainly concentrated on screening the high active constituent. By searching literatures of recent years, the current situation about the formulation of mosquito repellent was summarized, in order to provide scientific basis for the research and development of mosquito repellent formulation.%植物源驱避剂,具有安全、环保、毒副作用小等特点,目前对其研究重点在于筛选高驱避活性成分.本文通过查阅近年来的文献资料,对蚊虫驱避剂剂型的研究进展进行综述,为蚊虫驱避剂剂型的研究开发提供科学依据.
  • 摘要：Objective In recent years, with the worsening situation of the international nuclear crisis as well as people prolonged exposure to low doses of radiation in the surrounding environment in daily lives, the research on radio-protective drugs was much more crucial. Natural medicines and ethnic drugs were attended by researchers'eyes for their outstanding advantages such as rich resources , exactly curative effects and lower side effects. The research progress on common natural medicines and their active components as well as ethnic drugs that are usually used as anti-radiation drugs were reviewed in this paper.%近年来,国际核危机形势严峻以及日常生活中人们长期遭受周围环境中低剂量辐射,使辐射防护药物的研究显得至关重要.而天然药物以及民族药物由于其资源丰富、疗效确切、副作用小等优点,越来越受到研究者的重视.本文就常见的具有抗辐射作用的天然药物及其活性成分,以及疗效确切的抗辐射民族药物研究进展作一综述.
  • 摘要：抑郁症是一种常见的精神疾病,其发病率在不断上升,而且难以治愈.目前抑郁症的治疗药物主要包括:三环类抗抑郁药、单胺氧化酶抑制剂和可逆性选择性单胺氧化酶抑制药、选择性5 -HT再摄取抑制剂、选择性NE再摄取抑制剂、5-HT及NA再摄取抑制药、NA及DA再摄取抑制药等.本文就抗抑郁药及特殊情况下抑郁症的药物治疗进展作一综述.%Depression was a common mental disorder. Nowdays, anti-depressants included tricyclic antidepressants, mono-amine oxidase inhibitors and reversible selective monoamine oxidaae inhibitors, selective serotonin reuplake inhibitors, selective nora-drenergic reuptake inhibitors, serotonin and norepinephrine reuplake inhibitors, norepinephrine and dopamine reuptake inhibitors. The progress on drug treatment of depression were reviewed in this paper.
  • 摘要：目的 建立气相色谱-质谱法(GC-MS)定性分析麝香保心丸挥发性入血成分的方法.方法 健康雄性SD大鼠给予麝香保心丸混悬液灌服(剂量3.4 g/kg体重),于口服30 min后肝门静脉取血,蛋白沉淀法处理血浆样品进行GC-MS分析.分析条件:Thermo TR-5 ms色谱柱；Xcalibur色谱工作站；载气为氮气；载气流速为1.0 ml/min；进样口温度为220℃；离子源温度为250℃；梯度升温；进样体积为1 μ1;分析时间59 min.结果 通过比较麝香保心丸提取液、空白组及给药组,及与在线NIST05数据库比对,共识别和鉴定10个化学成分,其中6个为麝香保心丸中原型成分,其余4个为代谢物.结论 本研究建立了一种快速筛选麝香保心丸中挥发性入血成分的方法,可为其深入研究提供基础.%Objective To identify the volatile components in rat serum of Shexiang Baoxin Pill (SBP) by gas chromatography couples with mass spectrometry (GC-MS). Methods Serum samples from control and SBP administration rats were deproteinaled by adding methanol prior to gas chromatography. The separation was carried out on a Thermo TR-5 ms column (30 m × 0. 25 mm I. D. ). The inlet temperature was set at 220 ℃ and ion source temperature was 250 ℃ with gradient warming. The carrier gas was N2 with a gas flow rate of 1.0 ml/min, and the injection volume was 1μl. The total analysis time was 59min. The data acquisition were performed on Xcalihur workstation. Results Ten components including 6 components from SBP and 4 metabolites were observed from a comprehensive analysis of the chromatography of SBP solution. All the observed components were identified by using the online NIST05 library. Conclusion An integrated method for screening volatile bioaclive components in serum after oral administration of SBP was established, which provided helpful information for further mechanism research.
  • 摘要：Objective To review ihe role of Ribosomal Protein S3a in regulation of proliferation, differentiation and apoptosis in tumor cells. Methods The relevant literatures were collected to make a summary of Ribosomal Protein S3a in regulation of proliferation, differentiation and apoptosis in tumor cells. Result and Conclusion Ribosomal Protein S3a played an important role in protein synthesis and also had unique extra-ribosomal functions, such as DNA repair, cell growth and cell differentiation. In recent years, it was found that the expression of Ribosomal Protein S3a was enhancedin tumor cells. It was involved in the regulation of proliferation, differentiation, and apoptosis of tumor cells by affecting the expression of oncogenes and lumor suppressor genes.%目的 综述核糖体蛋白S3a在肿瘤细胞增殖分化和凋亡调控作用的研究进展.方法 参阅近几年国内外相关文献,对核糖体蛋白S3a的结构、功能及其异常表达对肿瘤细胞增殖分化和凋亡的调控等方面的进展进行归纳总结.结果与结论 核糖体蛋白S3a除了在蛋白质合成中起重要作用外,还有独特的核糖体外功能.其在多种肿瘤细胞中高表达,通过调控癌基因和抑癌基因的表达可影响肿瘤细胞的增殖分化与凋亡.
  • 摘要：目的 评价热毒宁注射液在儿科疾病中的临床疗效.方法 查阅近年来热毒宁注射液在儿科方面的相关文献进行分析.结果与结论 热毒宁注射液具有抗菌、抗病毒、提高免疫力的作用,在治疗急性上呼吸道感染、社区获得性肺炎、毛细支气管炎、疱疹性咽峡炎、急性化脓性扁桃体炎、轮状病毒性肠炎、手足口病、甲型H1N1流感、病毒性脑炎、流行性腮腺炎、水痘、麻疹等儿科疾病中疗效肯定,临床应用广泛.%Objective To evaluate the clinical effect of Reduning injection in the pediatric disease. Methods The pertinent literatures on pediatrician using Redlining injection in recent years were reviewed and analyzed. Results and Conclusion Reduning injection had antibacterial and antiviral effect, which could improve the immune system. Reduning injection obtained remarkable effect in the treatment of acute upper respiratory tract infection, Community-acquired pneumonia, capillary bronchitis, herpes angina, acute suppurative tonsillitis, rotavirus enteritis, hand-foot-mouth disease, influenza H1N1,viral encephalitis, epidemic parotitis, varicella and measles,popularize in the clinic.
  • 摘要：Objective To identify different breeds of Fagopyrum tataricum by differential thermal analysis and near infrared reflectance spectroscopy. Methods TG/DTA and thermal analysis were both used to scan and analyze the thermal map of 11 breeds of F. tartaricum. The experiment conditions included DTA range of ± 50 μV , a calefactive rate of 15 ℃ /min, an ambience of nitrogen and flow velocity of 50 ml/min. Bruker Vector 22/N-type Fourier transform near infrared spectroscopy was used to scan and analyze of 11 breeds F. tartaricum. Experiment conditions were; integrating sphere diffuse reflectance as measure methods; scan for 64 times; 3 700 - 12 000/cm as scan range. Results Both methods could identify the Jiande lartary buckwheat from other breeds. Conclusion Jiande tartary buckwheat was a new cultivar, nutrient value and pharmacological activity of which should be evaluated for promotion.%目的 用差热分析法(DTA)和近红外漫反射光谱(NIRS)法对11种栽培苦荞麦进行快速分析鉴别.方法 应用TG-DTA热重差热联用仪分别对11个栽培品种苦荞麦进行差热图谱扫描与分析.实验条件为差热量程:±50μV；升温速率:15℃/min；升温范围:35℃～500℃；气氛为氮气,流速:50 ml/min.应用Bruker 1111Vector 22/N型傅立叶变换近红外光谱仪对11种栽培苦荞麦进行扫描.实验条件为测定方式:积分球漫反射；扫描次数64次；扫描范围:3 700～12 000/cm.结果 结合两种方法能快速鉴别“建德苦荞麦”同其他苦荞麦,鉴别效果良好.结论 “建德苦荞麦”作为一个新的培育品种,可以对其营养成分、药理活性进行进一步评价,为广泛推广提供参考.
  • 摘要：目的 研究尼莫地平口腔崩解片在人体内的药动学,比较其与市售尼莫地平片人体内药物动力学及相对生物利用度.方法 将6名健康志愿者随机分成两组,交叉单剂量口服尼莫地平口腔崩解片A和市售片B各60 mg,采用HPLC 法测定血浆中尼莫地平的浓度.结果 口服尼莫地平口腔崩解片A和市售片B后,主要药代动力学参数分别为Cmax(256.23±54.64) g/ml、( 102.415±36.96) g/ml;Tmax分别为(0.7049±0 035)h、(1.3317±0.123) h;T1/2分别为(0.2734±0.031)h、(1.1958±0.141) h;AUC0-1分别为(622.77±98.03) g/(ml·h)、(354.63±96.24)g/(ml·h).尼莫地平口腔崩解片A相对于市售片B的人体生物利用度为177.27％.结论 自制尼莫地平口崩片速释效果明显,并能提高生物利用度.%Objective To research the pharmacokinetics and relative bioavailability of Nimodipine rapid disintegeating tablet in human body, which was compared with Nimodipine market tablets. Methods 6 cases of health volunteer were divide into two groups randomly , alternately taking rapid disintegrating tablet A and market tablet B of Nimodipine 60mg single dosage orally, HPLC was used to determine the concentration of Nimodipine in blood. Results The main Pharmacokinetics parameters after single oral administration Nimodipine rapid disintegeating tablet A and Nimodipine market tablet B were (256.23 ±54.64) g/ml, (102.415 ±36. 96) g/ml in Cmax (0. 7049 ±0. 035) h, (1. 3317 ±0. 123) h in Tmax; (0.2734 ±0.031) h, (1.1958±0. 141) h in T1/2; (622.77 ±98.03) g/(ml · h), (354.63 ±96.24) g/(ml · h) in AUC0-t, respectively. The bioavailability of rapid disintegeating tablet was 177.27% relative to market tablet. Conclusion Nimodipine rapid disintegealing tablet was capable of releasing rapidly, and had an enhanced bioavailahility as compared with market tablet.
  • 摘要：目的 探讨医院药库药品零库存模式下药房二级库的库存管理方法,发挥零库存管理优势,思考对策,确保临床用药.方法 介绍药库零库存管理模式,比较实施零库存管理前后,库存量、药品与资金周转率.结果 实施零库存管理后,医院药库库存药品品种从原来的1 100余种减少到现在的200余种,全院库存药品数量、金额明显下降,周转率大幅度提高,平均资金周转率从111.0％提高至220.3％,门诊药房平均资金周转率从136.5％提高至253.9％.结论 实施零库存管理后,库存结构优化,药品库存量减少,提高了药品采购效率和管理水平,减轻了医院运营成本.%Objective To discuss the managed method of second order drug storeroom inventory under "zero inventory" model, and take advantages of "zero inventory" management, find the counlermeaaure, and ensure clinic drug use. Methods The model of "zero inventory"was introduced, and compared the inventory quantity, drug varieties and capital turnover rate before and after that zero inventory management was implemented. Results After zero inventory management was implemented, the varieties of drug in our hospital reduced to 200 from original more than I 100, the drug inventory and the sum of money decreased obviously, the turnover rate rose substantially. Average capital turnover rate increased from 111 % to 220. 3% , and average capital turnover rate of outpatient pharmacy increased from 136. 5% to 253.9%. Conclusion After implement of zero inventory management, the structure of inventory was optimized, the drug amount of inventory was decreased, the efficiency of medicines purchase was increased, the level of management was improved and the cost of hospital working was reduced.
  • 摘要：目的 研究电子处方信息在门诊医师处方行为分析中的应用.方法 采集某区级中心医院消化内科2010年全年疾病诊断为消化道溃疡的电子处方信息进行分析,运用SPSS18.0进行统计检验和分析.结果 中级职称的医师处方总金额和处方中国产药品金额与初、高级职称相比存在显著性差异,自费患者的处方总金额、处方品种总数和国产药品金额均高于医保患者,处方总金额与患者费别、患者年龄段、处方中进口药品品种构成比、处方中自费药品品种构成比和金额构成比存在线性相关.结论 中级职称医师应当是该院门诊药品消费控制干预的重点人群,医保政策无法干预医师对自费药品的处方行为,电子处方信息可结合疾病诊治规范解释处方行为差异.%Objective To investigate the application of electronic prescriptions data in prescribing behavior research. Methods 2010 annual electronic prescription information diagnosis of peptic ulcer were collected in a district-level central hospital, SPSS (veraionl8.0 ) was used to statistical analysis. Results Total amount of prescription prescribed by attending physicians were significantly different with residencies and chief physicians; Total amount and the total number of prescription without medical insurance were higher than those with medical insurance; there was a linear relationship among prescription total amount and medical insurance, patient age, the number constituent ratio of imported drugs, the number constituent ratio of own-expense drugs, the amount constituent ratio of own-expense drugs. Conclusion Attending physicians should be the focus group by outpatient consumption control interventions, medical insurance policies could not restrict at own-expense medicines prescription, electronic prescriptions data could be used in prescribing behavior research combined with treatment guidelines.
  • 摘要：目的 提升医院门诊药房的管理水平.方法 基于原有医院信息系统,采用Power builder 6.5系统前台数据库开发工具及数据挖掘技术,根据实际情况不断完善门诊药房信息系统.结果和结论 提高了门诊药房的工作效率,缩短了患者的等候取药时间,而且大大地减少了差错的发生.%Objective To improve the level of management of outpatient pharmacy in our hospital. Methods Power builder 6.5 software and data-mining technology were applied based on original hospital information system, the outpatient pharmacy information system was continuously upgraded. Results and Conclusions The working efficiency of outpatient pharmacy was greatly improved by the information system. The patient's waiting time was shortened, and the incidence of error was greatly reduced.
  • 摘要：Objective To optimize the preparation and study in vitro releasing of SRL-NLC dispersion. Methods Central composite design-response surface methodology was used to optimize dispersion formulation,then in vitro releasing in 0.4% SDS(sodi-um lauryl sulfate) solution was explored by normal phase dialysis bag method. Results The optimal dispersion had PS (particle size) : 82.54nm,PI(polydisperity index) : 0. 207, Zeta potential: - 18. 0 mv, DL ( drug-loading) : 1.829%, and EE ( entrapment efficiency) : 91.3%; SRL-NLC dispersion had a characterization of 60. 1% accumulated releasing in 0.4% SDS solution. Conclusion The optimal formulation had good feasibility and reproducibility, in vitro releasing curve showed SRL-NLC dispersion had a characterization of 60. 1% accumulated releasing in 0.4% SDS solution.%目的 优化西罗莫司纳米结构脂质载体(sirolimus nanostructured lipid carriers,SRL-NLC)分散液的处方,并考察其体外释放度.方法 采用星点设计-效应面法(central composite design-response surface methodology,CCD-RSM)优化SRL-NLC分散液的处方,并以粒径、分布系数、载药量和包封率作为评价指标,采用正相透析袋法考察SRL-NLC分散液在0.4％SDS(十二烷基硫酸钠)溶液中的释放度.结果 经优化的SRL-NLC分散液平均粒径82.54 nm、分布系数0.207,Zeta电位-18.0mv、载药量1.829％和包封率91.3％,SRL-NLC分散液在0.4％ SDS溶液中能持续释放120 h,累积释放量为60.1％.结论 经优化的处方可行性和重现性均较好,SRL-NLC分散液在0.4％ SDS溶液中累积释放度为60.1％.
  • 摘要：Objective To investigate the effectiveness and safety of the inhalation of tiotropium bromide in the treatment of moderate to severe chronic obstructive pulmonary disease. Methods 35 cases with chronic obstructive pulmonary disease during 2010 June to 2011 November in our department were observed which was used by tiotropium bromide dry powder (Spirva, 18 u.g, once daily) . The pulmonary function, average daily use of beta agonists times and cardiopulmonary insufficiency in patients with Plasma brain natriuretic peptide levels( BNP)were compared before treatment and three months after the treatment. The number of admissions three months before treatment and three months after treatment was recorded,and the adverse reaction observed. Results Tiotropium pulmonary function was improved significantly, the number of daily use of beta agonists reduced, peripheral blood BNP decreased obviously, the number of acute exacerbation of seizures became less,no serious adverse reaction occurred. Conclusion Tiotropium bromide was effectiveness and safety in moderate to severe chronic obstructive pulmonary disease.%目的 探讨吸入噻托溴铵治疗中重度慢性阻塞性肺病的疗效和安全性.方法 观察2010年6月～2011年11月门诊或住院使用噻托溴铵干粉剂(思力华,18μg,1次/d)的慢性阻塞性肺病患者35例.比较患者治疗前及治疗3个月后肺功能、平均每日使用β受体激动剂次数及心肺功能不全患者外周血浆脑纳肽水平(BNP),记录患者治疗前3个月及治疗后3个月因病情加重入院治疗的次数,同时观察不良反应.结果 吸入嚷托溴铵后肺功能明显好转,每日使用β受体激动剂次数减少,BNP水平有明显下降,急性加重发作的次数减少,无严重不良反应发生.结论 噻托溴铵对于中重度慢性阻塞性肺病患者疗效显著且安全可靠.
  • 摘要：Objective To establish and optimize a simple, rapid, reliable, high sensitive and high selective method for the determination of fluoxetine hydrochloride in plasma by high-performance liquid chromatographic (HPLC) so as to monitor the clinical drug use. Methods The plasma samples were deproteined by acelonitrile. Separation was performed on a Hypersil OD52 column (5 μm, 4.6 mm × 200 mm). The mobile phase contained acelonitrile-25 mmol/ L phosphate buffer ( pH6. 5 ) (34 : 66). The flow rate and sample volume injected were 1.2 ml/min and 20 μl, respectively. Detection wavelength was 227 nm. Results The calibration curve of fluoxetine hydrochloride in plasma was linear in the concentration range of 0. 5 -50 ng/ml and coefficient was 0. 996 9. The intra-assay precision did not exceed 8.06% and inter-assay precision did not exceed 10.46% for low, medium and high quality samples, respectively. The average recovery of the described method was 91.7% ,97.06% and 100.75% , respectively. Conclusion In comparison with previous work, the improved chromatographic method was more economical, less polluted and better protected column, besides simple preparation of plasma samples, high reproducibility, and high selectivity.%目的 建立并优化一种简单、快速、灵敏及高选择性测定氟西汀血药浓度的高效液相色谱法(HPLC),用于临床个体化给药监测.方法 血浆样品用乙腈沉淀蛋白法处理.样品分离用Hypersil ODS2(5μm,4.6 mm×200 mm)色谱柱,流动相为乙腈-25 mmol/L磷酸盐缓冲液(pH6.5)(34∶66),流速为1.2 ml/min；进样量为20μl,紫外检测波长227 nm.结果 氟西汀的血浆浓度范围是0.5 - 50 ng/ml,r =0.996 9,低、中、高血浆质控样品日内RSD均小于8.06％,日间RSD均小于10.46％,方法平均回收率分别为91.7％,97.06％和100.75％.结论 与已报道的氟西汀血药浓度测定方法相比,本方法血浆样品处理简单,重现性高,选择性好,更经济、污染少且能更好地保护色谱柱.
  • 摘要：通过与DNA的结合而从生物学源头阻止疾病的发生成为近年研究的热点,DNA G-四链体结构的发现以及分子生物学相关技术对其与癌症关系的揭示,为抗肿瘤药物的设计提供了一个良好的突破口.本文综述了近三年来G-四链体的结构及其与小分子配体成复合物的结构研究进展,有助于理解G-四链体与小分子配体的作用模式,以期为合理设计抗肿瘤药物有所帮助.%There was a research focus for the prevention of diseases by way of binding 1o DNA using small molecules. The discovery of structures of G-quadruplex and Us function related to cancer through modern molecular biology techniques provided a big chance for the design of anii-cancer drug. This article aimed to present recent advances for the slructures of G-quadruplex and these small binding molecules, which would contribute to understand these binding modes and thus provide a steady support in the rational design of anti-cancer drug.
  • 摘要：目的 运用GC-MS对不同提取方法所得的艾叶挥发油成分进行比较分析.方法 分别采用水蒸气蒸馏,正己烷回流,静态顶空提取艾叶挥发油,运用气相色谱-质谱联用技术,结合NIST05数据库对分离的化合物进行鉴定,并应用峰面积归一化法计算各成分的相对含量.结果 3种方法共鉴别出艾叶中80个挥发油成分,其中静态顶空进样59个,水蒸气蒸馏37个,正己烷回流31个.结论 结合水蒸气蒸馏和静态顶空进样两种方法可以建立更全面的艾叶挥发性成分GC-MS表征体系；静态顶空进样在挥发油成分鉴别上存在一定优势.%Objective To analyze the the compositions of volatile oil from Artemisia argyi Levi. et Vant extracted by three different methods by GC-MS. Methods The volatile oil from Artemisia argyi Levi. et Vant were extracted by Steam distillation , Hexane reflux extraction and Static headspace. GC-MS was used for the separation and identification. Results By the three methods, 80 compounds were identified. 59 components were extracted by static headspace, 37 by steam distillation and 31 by hexane reflux extraction. Conclusion Combination of steam distillation and static headspace, sampling was feasible to establish GC-MS representation system for Artemisia argyi Levi. et Vant; there were certain advantages of static headspaee in determination of volatile oil contant.
  • 摘要：Objective To improve the preparation process of tamibarotene. Methods 1 ,2 ,3 ,4-tetrahydro-l , 1,4 ,4-tetram-ethyl-6-acetylaminonaphthalimide was prepared from 2 ,5-dimethyl-2 ,5-hexanediol by chloridized and Friedel-Craft reaction with acetan-ilide, which was subjected to deacylation, acylation and hydrolyzation to give tamibarotene. Results The process was successfully improved due to low cost, easier post-processing and good yield. The purity of the product was high (＞99. 7% ) and no impurity more than 0. 1% . Conclusion The total yield of the improved process was 26.6% , which was more suitable for industrial manufacturing. The structure of temibarotene was confirmed by 'H-NMR, ES1-MS and elementary analysis.%目的 改进他米巴罗汀的合成工艺.方法 以2,5-二甲基-2,5-己二醇为起始原料经氯代后与乙酰苯胺进行Friedel-Crafts环合反应制得1,2,3,4-四氢-1,1,4,4-四甲基-6-乙酰氨基萘,再经脱乙酰基、酰化、水解共5步反应合成了抗白血病药物他米巴罗汀.结果 优化后的工艺成本低、后处理容易、产物纯度高(纯度＞99.7％,单个杂质＜0.1％)、收率高.结论 新工艺的总收率达到26.6％,且适合工业化生产.目标产物结构经1H-NMR,ESl-MS和元素分析确证.
  • 摘要：Objective To explore the effect of valsartan on patients with hypertension and non-alcoholic fatty liver disease ( NAFLD). Methods A total of 148 patients suffered hypertension and NAFLD were included in the study. Patients were divided into the observed group the compared group, which were given valsartan and amlodipine respectively. The difference of the blood pressure, liver ultrasound, liver function, serum lipids, insulin resistance index ( RI ) and clinical curative effect were compared between the two groups. Results The curative effect in the observed group (93.24% ) was significantly higher than that in the compared group (81.08% ) ; After treatment, the alanine aminotransferase, cholesterol, triglyceride, low density lipoprotein and RI in the observed group were significantly lower than that in the compared group(P＜0. 05＝. Conclusion The valsartan had a good effect on patients suffered hypertension and NAFLD.%目的 探讨缬沙坦胶囊治疗伴有非酒精性脂肪肝( NAFLD)的高血压患者的疗效.方法 将符合纳入标准患者随机分成试验组和对照组.试验组给予缬沙坦胶囊；对照组给予氨氯地平片.比较两组血压、肝脏超声、肝功能、血脂、胰岛素抵抗指数(RI)的差别及临床疗效.结果 缬沙坦胶囊组总有效率(93.24％)显著高于对照组(81.08％),差异有统计学意义(x2=4.891,P=0.027)；治疗后,试验组谷丙转氨酶、胆固醇、甘油三酯、低密度脂蛋白及RI均显著低于对照组,差异有统计学意义(P＜0.05).结论 缬沙坦胶囊治疗伴有NAFLD的高血压患者具有更好疗效.
  • 摘要：Objective To establish a high performance liquid chromalography method for the determination of buddleoside in Zhikuoyangyin granules. Methods The HPLC method was performed on a DIKMA Platisil ODS column(250 mm×4.6 mm,5μm) with the mobile phase consisting of methanol-waler-acetic acid(55:44:2). The flow rate was 1.0 ml/min. The sample injection volume was 20 μl. The detective wavelength was 326 nm. Results The calibration curve of buddleoside showed good linear response ranged from 2. 95 to 98. 3 μg/ml with r= 0.999 9(n=5). The average recovery of spiked samples for buddleoside was 100.2% while the relative standard deviation for repetitions was 0. 96% ( n = 15 ). Conclusion The method was simple, sensitive, reliable and re-peatable, and could be used for the quantitative determination of buddleoside in Zhikuoyangyin granules.%目的 建立HPLC测定支扩养阴颗粒中蒙花苷的含量方法.方法 色谱柱为DIKMA Platisil ODS柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-醋酸(55∶44∶2),流速1.0 ml/min,进样量20μl,检测波长326 nm.结果 蒙花苷在2.95～98.3 μg/ml内线性良好,r=0.999 9(n =5),平均回收率100.2％,RSD为0.96％(n=15).结论 该法操作简便、灵敏、可靠,可用于支扩养阴颗粒中蒙花苷的含量测定.
  • 摘要：Objective To find out the best prescription method for Yantongping transdermal ointments through orthogonal design and research on the effect of promoting blood circulation. Methods With 60 minutes in vitro cumulative penetration rate, using L9(34) orthogonal experiment, the best formula and technology were found out through grinding method, and quality thereafter was e-valuated. Mice was picked up as blank groups and model groups, each respectively was divided into six smaller groups as Vaseline, Vaseline and Fatty Alcohol, Yantongping transdermal ointments( low dosage) , Yantongping transdermal ointments( medium dosage) , Yantongping transdermal ointments( high dosage) and Voltaren Emulsions. A A and AV were measured before and after modeling by microcirculation method and microscopic micro-distance measuring method , while the blood circulation function of each medicine by the scoring of the blood of veinule was compared. Results The best prescription was confirmed by A2B2C3D2, that was with the weight of the traditional Chinese drugs (TCD) Vaseline ratioes ( A) to 50% , with the weight of TCD and Fatty Alcohol ratios ( B) to 25% , the order of reagent was as follow; Fatty Alcohol, TCD, Vaseline . The grinding temperature was 80 X. , and it passed five quality evaluation as physical appearance, melting point, water value, stability, irritation. Before modeling, in the A A level measured, Yantongping group (low dosage) had statistical significance over Vaseline and Fatty Alcohol group in 30 min(P＜0.05＝ ,while Yantongping group (medium dosage and high dosage) had obviously statistical significance over Vaseline and Fatty Alcohol group in different time periods in the A A and A V level (P＜0. 01＝. In the blood flow quality evaluation measured, Yantongping group (low dosage) had statistical significance over Vaseline and Fatty Alcohol group in 20 min, 30 min ( P＜0. 05 ) ; while Yantongping group ( medium and high dosage) had obviously significant statistical significance over Vaseline and Fatty Alcohol group(P＜0.01 ). After modeling, in the A A level, Yantongping group (low dosage) had statistical significance over Vaselineand and Fatty Alcohol group in 20 min(/) ＜0.05) ; Yantongping group (low dosage) in 30 min, Yantongping group (medium and high dosage) had obviously significent statistical significance over Vaseline and Fatty Alcohol group in the A A and AV level. In the blood flow quality evaluation measured, Yantongping group (low,medium and high dosage) had obviously significant statistical significance over Vaseline and Fatty Alcohol group (P＜0.01). Conclusions Yantongping transderraal ointments prepared by the best prescription had a good function of promoting blood circulation.%目的 通过正交试验筛选出最佳配方及工艺,制备炎痛平透皮软膏剂,探讨其活血作用.方法 以60 min体外累积透皮速率为指标,采用四因素三水平L9(34)正交试验,通过研和法筛选出最佳配方及工艺,并对其进行质量评定.将小鼠分为空白对照组与模型组,各组再分为6组,每组各6只,组别分别为:凡士林组,凡士林+脂肪醇组,小、中、大剂量炎痛平组和双氯芬酸二乙胺(扶他林)组.用微循环障碍法与显微测微法,在不同时间测量各组动物造模前与造模后的微动、静脉管径的差值(△A、△V),同时对微静脉血液的流态进行评分,作为观察指标,比较各组药物活血作用的差异.结果 确定炎痛平透皮软膏剂最佳配比及工艺为A2B2C3D2,即凡士林与中药的重量比例(A)为50％、脂肪醇与中药的重量比例(B)为25％、研和温度(C)为80℃,加入顺序为:脂肪醇、中药、凡士林.并通过了物理外观、酸碱度、熔点、稳定性与刺激性等5项质量评定.造模前,测得小剂量炎痛平组在30 min时△A值大于凡士林+脂肪醇组,其差异有统计学意义(P＜0.05)；中剂量与大剂量炎痛平组在各个时间段测得△A、△V值均大于凡士林+脂肪醇组,其差异有统计学意义(P＜0.01)；小剂量炎痛平组在20、30 min时测得血流流态评分值均大于凡士林+脂肪醇组,其差异有统计学意义(P＜0.05)；中剂量与大剂量炎痛平组在各个时间段测得血流流态评分值均大于凡士林+脂肪醇组,其差异有统计学意义(P＜0.01).造模后,小剂量炎痛平组在20min时测得△A值大于凡士林+脂肪醇组,其差异有统计学意义(P＜0.05)；小剂量炎痛平组在30 min时,中剂量与大剂量炎痛平组在各个时间段测得△A、△V值均大于凡士林+脂肪醇组,其差异有统计学意义(P＜0.01)；小剂量、中剂量与大剂量炎痛平组在各个时间段测得血流流态评分值均大于凡士林+脂肪醇组,其差异有统计学意义(P＜0.01).结论 以最佳配方与工艺制备的炎痛平透皮软膏剂具有较显著活血作用.
  • 摘要：目的 研究金樱子Rosa Laevigata.Michx.嫩叶的化学成分.方法 运用硅胶柱色谱、凝胶柱色谱(Sephadex LH-20)以及半制备高效液相色谱等多种现代色谱技术进行分离纯化,根据理化性质和波谱分析鉴定化合物结构.结果 从金樱子叶的70％乙醇提取物乙酸乙酯部位分离鉴定得到12个化合物,分别为儿茶素(1)、槲皮素(2)、柚皮素(3)、山柰酚(4)、没食子酸乙酯(5)、对羟基肉桂酸乙酯(6)、ω-hydroxypropioquaiacone(7)、3,4-二羟基苯乙醇(8)、邻羟基苯甲酸(9)、莨菪亭(10)、乙基-α-D-呋喃阿拉伯糖苷(11)、甲基-O-β-D-葡萄糖苷(12).结论 化合物11为首次从蔷薇属植物中分离得到,化合物3、5～9为首次从金樱子叶中分离得到.%Objective To investigate the chemical constituents from leaves of Rosa Laevigata Michx. Methods The chemical constituents were isolated and purificated by silica gel column chromatography, Sephadex LH-20 and semi-preparative RP-HPLC. Their structures were identified by spectral data together with physicochemical analysis. Results Twelve known compounds were isolated and identified as catechin (1) , quercetin (2) , naringenin ( 3 ) , kaempferol ( 4) , ethyl gallate ( 5 ) , p-hydroxyl ethyl cinnamate ( 6 ) , co-hydroxypropioquaiacone(7 ) , hydroxytyrosol(8) , o-hydroxybenzoic acid(9) ,scopoletin( 10) .ethyl a-D-arabmofuranoside( 11 ) , methyl f}-D-glucopyranoside( 12). Conclusion Compound 11 was isolated from genus Rosa for the first time, compounds 3, 5 ~9 were isolated from leaves of Rosa Laevigata Michx for the first time.
  • 摘要：Objective To confirm the effect of interval usage of furosemide and torasemide in treatment of chronic heart failure (CHF). Methods By simple randomization, the patients were divided into the observe group (interval usage of furosemide and torasemide ) and the control group( continuous usage of furosemide). The difference of the efficiency, urine volume, electrolytes and pro-BNP were compared between the two groups. Results The efficiency, urine volume and serum potassium were significantly higher in the observed group than that of the control group( P＜0.05＝. The pro-BNP and the incidence of adverse reactions were significantly lower in the observed group than that of the control group (P＜0.05＝. Conclusion The interval usage of furosemide and torasemide could get better curative effect in treatment of CHF.%目的 探讨呋塞米与托拉塞米间隔使用治疗慢性心力衰竭(CHF)的疗效.方法 将符合纳入标准患者随机分成试验组和对照组.试验组先使用呋塞米片7d,然后改为托拉塞米片7d；对照组使用呋塞米片14 d.比较两组治疗14d后总有效率、尿量、电解质及pro-BNP的差别.结果 ①试验组总有效率为89.66％,显著高于对照组(77.01％),差异有统计学意义(x2=7.968,P=0.019)；②两组治疗后尿量较治疗前显著增加,差异有统计学意义(P＜0.05),血钾和pro-BNP均较治疗前显著下降,差异有统计学意义(P＜0.05)；③治疗后试验组尿量和血钾显著高于对照组,差异有统计学意义(P＜0.05),pro-BNP显著低于对照组,差异有统计学意义(P＜0.05)；④试验组总不良反应发生率及低钾血症发生率显著低于对照组,差异有统计学意义(P＜0.05).结论 呋塞米与托拉塞米间隔使用可提高CHF治疗的总有效率,增加尿量,减少电解质紊乱等不良反应.
  • 摘要：Objective To investigate the effects of Fe3+ on the stability of ornidazole and sodium chloride injection,and ensure the safety in clinical utility. Methods Two kinds of ornidazole and sodium chloride injection which contain no Fe3+ and trace Fe3 + were prepared respectively. The content of ornidazole was determined by HPLC at different temperatures and times, which was used to study chemical dynamics of ornidazole . According to Arrhenius equation, stability of two kinds of ornidazole and sodium chloride injection at room temperature were forecasted. Results The degradation of ornidazole followed the first-order reaction kinetics model. At 25?, t0.9 of ornidazole and sodium chloride injection which not containing Fe3+ and containing trace Fe3+ were 1.71 years and 1.11 years separately. Conclusions Trace Fe3+ could accelerate the degradation of ornidazoie, which shortened storage period of ornidazole and sodium chloride injection.%目的 考察铁离子对奥硝唑氯化钠注射液稳定性的影响,保证制剂临床用药安全.方法 分别制备不含铁离子及含微量铁离子的奥硝唑氯化钠注射液,用HPLC方法测定两种奥硝唑氯化钠注射液在不同温度不同时间的奥硝唑含量,研究奥硝唑在不同温度下的化学降解动力学,运用Arrhenius方程,预测两种奥硝唑氯化钠注射液在常温下的稳定性.结果 奥硝唑氯化钠注射液中奥硝唑的降解符合一级动力学模型,25 ℃时,不含铁离子的奥硝唑氯化钠注射液的t0.9为1.71年,含微量铁离子的奥硝唑氯化钠注射液的t0.9为1.11年.结论 微量铁离子会加速奥硝唑分解,致使奥硝唑氯化钠注射液贮存时间变短.
  • 摘要：目的 建立同时测定谷光甘肽原料及制剂中氧化型和还原型谷光甘肽的反相高效液相色谱法.方法 采用外标法,以Hypersil C18为色谱柱,25 mmol/L KH2PO4(pH2.5)∶四氢呋喃(100∶1)为流动相,流速为1.0 ml/min,柱温为30℃,检测波长230 nm,进行分离测定.结果 在3.12 ～2 490.0μg/ml间,GSH线性关系良好(r=0.999 9),测得回收率为101.84％.在3.25～ 975.0μg/ml间,GSSG线性关系良好(r=0.9999),测得回收率为97.84％.结论 所用方法准确可靠、专属性强,可用于GSH和GSSG的同时测定.%Objective To establish a method to determine GSH and GSSG by HPLC. Method The analytical column was Hypersil C18.The mobile phase consisted of 25m mol/L KH2PO4 ( pH2. 5 ) :tetrahydrofuran( 100 :1 ). The flow rate was 1.0 ml/ min. Column temperature was 30℃ and the detective wavelength was 230 nm. Result The calibration curve of GSH was linear within the range of 3. 12 |xg/ml ~2490. 0 μg/ml(r =0. 999 9) and the average recovery was 101. 84% . The calibration curve of GSSG was linear within the range of 3. 25 μg/mi～975. 0 u,g/ml( r = 0. 999 9) and the average recovery was 97. 84% . Conclusion The method was proved to be reliable and accurate, which could be used to determinate GSH and GSSG simultaneously.
  • 摘要：The characterises of experiment in pharmaceutical specialties and the current situation that some experimental teaching courses were fragmented in pharmaceutical specialties were analyzed in this article. According to the general rules of the drug research and development, namely the raw materials-preparation-quality evaluation, the experiment content was made up by two parts: pharmaceutical foundational experiment and pharmacy comprehensive and designing experiment. By studying the whole process of the API to the finished product medicine. students could learn to use the modern detection means and method, take scientific evaluation for quality of API and preparation medicine, drug safety and effectiveness and learn how to get safe and effective drugs.%本文分析了药学专业实验的特点和当前药学专业实验教学部分课程各自为政的情况,根据药物研制与开发的一般规律,即原料-制剂-品质评价,将实验内容组建为两大模块:药学基础实验和药学综合性与设计性实验,通过从原料药到成品药的全过程学习,使学生学会采用现代检测分析手段与方法,对原料药与制剂药的质量以及药物的有效性、安全性进行科学评价,从而学习如何获得安全有效的药物.
  • 摘要：目的 建立药学专业实验课研究性教学策略,提高学生的实践能力和创新精神,培养基础研究、新药研制和生产经营的复合型药学人才.方法 采用调查研究、文献研究和实验研究等方法.结果 设置了药学专业实验课研究性教学的实验内容、构建了开放教学的形式,建立了研究性教学的平台.结论 取得了较好的应用成效.%Objective To build up the teaching tactics of research in experiment course for pharmaceutical specialties, which could promote the ability of practicing and innovation, and cultivate the talents combined with fundamental research, new drug development and production management. Methods Experimentation, investigation, operation and literature research methods were used. Results Teaching contents, organization forms and teaching platform of research teaching in experiment course for pharmaceutical specialties were built up. Conclusion A marked teaching effects were achieved.
  • 摘要：Objective To introduce the progress on synthesis and biological activity of 2-pyridyl ketone derivatives. Methods The information described in literature both at home and abroad was reviewed. Results and Conclusion Based on the results of existed research , deep study on the mechanism of action and the targets would further promote the development process of these compounds.%目的 介绍2-吡啶酮类化合物的生物活性以及合成方法方面的研究进展.方法 查阅国内外文献资料进行分析归纳与整理.结果与结论 在现有研究成果的基础上,深入对该类化合物在作用机制以及作用靶点方面进行研究将会进一步推动该类化合物的研发进程.

    药学实践杂志的期刊信息

    • 曾用名：药学情报通讯
    • 创刊时间：1983
    • 地区：CN
    • 语言：中文
    • 热门主题：海军军医大学
    • 学科分类：

    药学实践杂志的获奖情况

    • • 2018年度中国高校优秀科技期刊
    • • 2016年度中国高校编辑出版质量优秀科技期刊
    • • 第三届上海市高校优秀科技期刊奖（2018）
    • • 第二届上海市高校特色科技期刊奖（2016）

    药学实践杂志的收录情况

    • • 化学文摘（网络版）
    • • 日本科学技术振兴机构数据库
    • • 中国科技核心期刊

    药学实践杂志的投稿信息

    • 投稿方式：在线投稿
    • 期刊官方网站：点击前往
    • 通讯地址：上海市国和路325号
    • 邮编：200433
    • 联系电话：021-81871322 81841324
    • 邮箱：yxsjzzs@163.com
    • 期刊栏目：综述；论著；研究报告；药物与临床；药事管理；专题论坛；专家论坛
    免费订阅本刊

    客服邮箱：kefu@zhangqiaokeyan.com

    京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有 出版物经营许可证

    • 客服微信

    • 服务号