不同产地个青皮高效液相色谱指纹图谱研究

摘要

Objective To develop a high performance liquid chromatography( HPLC ) fingerprint of Fructus citri immaturu from different regions. Methods The chromatographic column was Aichrom Bond-raising AQ C18( 250 mm×4.6 mm,5 μm ). The mobile phase was acetonitrile and 0. 1％ phosphoric acid water with gradient elution. Flow rate was 1.0 mL · min-1 at 30 ℃ and detection wavelength was 360 nm. Results The common mode of HPLC fingerprint was developed and quality of Fructus citri immaturu from different regions was similar. Conclusion This method is accurate and reproducible, which can be used for the quality control of Fructus citri immaturu.%目的 研究不同产地个青皮的高效液相色谱(HPLC)法指纹图谱.方法 用Aichrom Bond-AQ C18色谱柱(250 mm×4.6 mm ,5 μm),乙腈和0.1%磷酸水为流动相,采用梯度洗脱,流速:1.0 mL·min-1,柱温:30 ℃,检测波长:360 nm.结果 建立HPLC指纹图谱共有模式,各产地个青皮药材质量有较好的相似性.结论 该指纹图谱方法准确可靠,重复性好,可作为个青皮药材内在质量评价的依据.