天山花楸叶中多糖的含量测定及抑菌作用研究∗

摘要

Objective To establish a method for determination of polysaccharide from the leaves of Sorbus tianschanica Rupr.and its bacteriostatic action. Methods Phenol-sulfuric acid method was used for extraction and determination of polysaccharides in Sorbus tianschanica Rupr. Its antibacterial action was studied by control experiment. Results Average content of polysaccharides in Sorbus tianschanica Rupr was 2.56%.The polysaccharides exerted bacteriostatic effect, especially on Bacillus subtilis. Conclusion The method is simple and accurate, and can provide theoretical basis and reference for relative research of Sorbus tianschanica Rupr.%目的：测定天山花楸叶中多糖的含量并研究其抑菌作用。方法采用苯酚-硫酸法提取天山花楸枝叶中多糖，并测定多糖的含量，采用对照实验研究天山花楸多糖的抑菌作用。结果天山花楸枝叶中多糖的平均含量为2.56％；天山花楸枝叶中多糖具有一定的抑菌作用，且对枯草杆菌的抑菌作用较明显。结论该方法简便，结果准确，为进一步研究天山花楸枝叶提供实验依据和参考价值。