目的 观察阿魏酸钠对神经病理性疼痛大鼠脊髓中Bcl-2和Bax表达的影响,探讨阿魏酸钠对脊髓的保护作用.方法 成年雄性Wistar大鼠随机分为两组:A组(n=30)为生理盐水组,结扎左侧坐骨神经后每天腹腔注入2ml生理盐水；B组(n=30)为阿魏酸钠组,结扎坐骨神经后向腹腔注入2ml阿魏酸钠,100mg.kg-1·d-1,连续7d.分别在术前及术后6h、1d、3d、7d进行行为学测定,记录缩足阈值,检测脊髓中Bcl-2和Bax的表达,采用TUNEL法观察脊髓神经元凋亡.结果 两组大鼠坐骨神经结扎后均可诱发出神经病理性疼痛；凋亡神经元和Bcl-2和Bax阳性细胞均于术后6h开始出现,并于术后1d开始迅速增加,术后3d达峰值.与生理盐水组相比较,阿魏酸钠组大鼠脊髓组织中神经细胞凋亡指数和Bax表达水平显著降低,而Bcl-2的表达水平却显著增加(P＜0.05).结论 阿魏酸钠可能通过促进Bcl-2表达,抑制Bax表达,从而抑制了大鼠脊髓神经元凋亡,对脊髓有保护作用.%Objective To observe the influence of sodium ferulate (SF) on the expression of Bcl-2 and Bax in spinal cord neurons in rats with neuropathic pain, and to study the protective effects of SF on the spinal cord neurons in rats. Methods Sixty healthy adult male wistar rats were simply randomized into two groups: group A (n=30) and group B (n=30). The left sciatic nerves of the rats were ligated under chloral hydrate anesthesia, then the rats were given 2 ml normal saline (group A) and 100 mg ? Kg-1 ? D-1 SF (group B), which was dissolved in 2 ml normal saline respectively after operation. All the patients were tested for the time of paw withdraw by using the von Frey test before surgery, 6 hours, 1 day , 3 days, and 7 days after surgery. Apoptosis of spinal cord neurons was detected with the TU-NEL method. Changes of Bcl-2 and Bax expression in the spinal cord were detected through the immunohistochemi-cal methods and image analysis. Results Mechanical allodynia developed at 6 hours after sciatic nerve ligation. The expression of neuronal apoptosis, Bcl-2 and Bax were first detected in spinal cord at 6 hours after injury and thereafter, reaching the peak at 3 days. The spinal neuronal apoptotic index and the expression of Bax in group B was significantly lower than that in group A. However, the expression of Bcl-2 in group B was significantly higher than that in group A (P<0.05). Conclusion Through promoting the expression of Bcl-2 and inhibiting the expression of Bax, sodium ferulate can effectively inhibit spinal neuronal apoptosis and protect spinal cord neurons in rats,
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