A Preliminary Study of the Influence of T-2 Toxin on the Expressions of Bcl-2 and Bax of Ameloblasts in Rat's Incisor

摘要

To investigate the effects of T-2 toxin on the protein expression of Bcl-2/Bax of ameloblast in rat's incisor. 30 Wistar rats were divided randomly into 3 groups: control, low dose and high dose T-2 toxin poisoning group. After 4 weeks treatment, immunohisto-chemistry was used to observe the relative intensity of Bax and Bcl-2 expression in the ameloblasts. Quantitative analysis of the expression of Bax and Bcl-2 was performed with a computer image analysis system. Quantitative analysis showed the Bax protein expression was up-regulated, the Bcl-2 protein expression was down-regulated, and the Bax/Bcl-2 expression ratio was up-regulated in the secretory-stage ameloblasts treated with T-2 toxin, and this was significant between three groups (P ＜ 0.05). Thus, T-2 toxin can decrease the expression of Bcl-2 and increase the expression of Bax in the secretory-stage ameloblasts of rat's incisor. With the increase of T-2 toxin exposure dose, this effect is much marked.