Zn^2＋和Cd^2＋单独及联合作甩对青蛤消化酶活性的影响

摘要

Enzymatic method for the analysis of two metal ions separate and combined effects of clam protease, amylase and cellulase activity of the two separate and combined effects of metal ion concentrations were set at 10rag / L, 20rag / L, 40rag / L and 80rag / L, exposure for 4 days. The results show that clam three kinds of digestive enzymes amylase size〉 pro. tease〉 cellulase; set exposure concentrations in the experimental range, Zn2＋, Cd=＋ separate and combined effects of clam pro- tease, amylase and cellulose enzyme activity were a significant impact. Which, 40rag / L of Zn2＋ significant protease activa- tion, throe kinds of high concentrations of Zn2＋ and two kinds of intermediate concentrations of Zn2＋ Cd2＋ cellulase showed significant activation, and the rest am for the three clam showed inhibition of digestive enzymes, and different concentrations of metal ions in those three kinds of inhibition of digestive enzymes showed a different trend.%以酶学分析的方法研究了两种金属离子单独及联合作用对青蛤蛋白酶、淀粉酶和纤维素酶活力的影响，两种金属离子单独及联合作用时的浓度分别设置为lOmg／L、20mg／L、40mg／L和80mg／L，染毒时间为4天。实验结果表明，青蛤3种消化酶活力大小为淀粉酶〉蛋白酶〉纤维素酶；在实验设置的染毒浓度范围内，Zn^2＋、Cd2＋单独及联合作用对青蛤的蛋白酶、淀粉酶和纤维素酶活力均产生显著影响。其中，40mg／L的Zn^2＋对蛋白酶有显著的激活作用，3种较高浓度的Zn^2＋及2种中间浓度的Zn^2＋＋Cd^2＋对纤维素酶表现出显著的激活作用，其余的均对青蛤的这3种消化酶表现为抑制作用。且不同浓度的金属离子对这3种消化酶的抑制作用表现出不同的变化趋势。