火炭母提取物抑制人结肠癌HCT-116细胞体外增殖研究

摘要

目的 研究火炭母提取物对人结肠癌HCT-116细胞的体外增殖抑制作用和诱导凋亡作用.方法 MTT法筛选火炭母各部分提取物对HCT-116细胞的增殖抑制作用及活性作用部位;于荧光显微镜下采用Hoechst 33258凋亡染色法检测细胞凋亡形态变化;通过Annexin V-FITC/ PI双标记法于流式细胞仪检测细胞凋亡率.结果 火炭母乙酸乙酯供试液对HCT-116的细胞增殖抑制作用较强且呈剂量和时间依赖关系,作用48小时后其IC50值为120.04 mg/L;凋亡染色显示有细胞凋亡形态变化;流式细胞仪检测火炭母乙酸乙酯供试液能诱导HCT-116细胞凋亡并呈剂量依赖关系.结论 火炭母乙酸乙酯供试液能抑制人结肠癌HCT-116细胞增殖并诱导细胞凋亡.%Objective To study the effect of extracts from Polygonum chinense on the proliferation inhibitory and apoptosis-inducing of human colon cancer HCT-116 cells in vitro. Methods MTT assay was applied to screen the proliferation inhibitory rate of the extracts of Polygonum chinense on HCT-116 cell lines in vitro. The morphology of apoptotic cells was observed with Hoechst 33258 fluorescence staining. Flow cytometry was applied to detect apoptosis rate of HCT-116 cell and Annexin V-FITC/ PI double staining was used. Results Ethyl acetate part of Polygonum chinense had relatively strong inhibitory effect on HCT-116 cell lines in a dose and time-dependent manner,its IC50was 120.04 mg/L at 48 h. Apoptotic staining showed the morphological changes of apoptosis. The Flow cytometry showed that cell apoptosis could be reduced by ethyl acetate part of Polygonum chinense and the relationship was in a dose-dependent manner. Conclusion Ethyl acetate part of Polygonum chinense can inhibit the cell proliferation and can induce apoptosis of HCT-116 cell lines in vitro.