ADJUVANT FOR RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO, METHOD FOR RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO, METHOD FOR GROWTH FACTOR HARVESTED FROM RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO AND USE THEREOF

摘要

An adjuvant for rapid proliferation of human mesenchymal stem cells in vitro is provided to overcome the problem of low cell amplification efficiency of human mesenchymal stem cells in a culture process. The adjuvant added for the culture of human mesenchymal stem cells includes at least one antioxidant, and a basic fibroblast growth factor (FGF-2). The adjuvant is added into a medium containing the human mesenchymal stem cells, and the culture takes place in a normal oxygen environment (21% oxygen tension), and the cells are divided rapidly, and the cell cycle at synthesis phase (S phase) percentage is increased to reduce ageing and improve differentiation potential. The adjuvant not only amplifies human mesenchymal stem cells rapidly to harvest the growth factor, but also maintains the characteristics of the multifunction of stem cells for the purposes of culturing and amplifying the human mesenchymal stem cells.