顶空固相微萃取和同时蒸馏萃取应用于GC-MS分析野生刺梨汁挥发性成分的比较研究

摘要

Head-space solid phase micro-extraction method（HS-SPME） and simultaneous distillation extraction method（SDE） were compared for their sampling effectiveness in GS-MS analysis of volatile components in chestnut rose juice.Totally 52 compounds were identified,including 17 hydrocarbons,12 esters,7 alcohols,5 acids,3 phenols,3 ketones,3 aldehydes and 2 heterocycles.Thirty-seven volatile components were identified by HS-SPME method and 19 by SDE method.Compared to SDE method,HS-SPME method has the advantages of rapidity,simplicity,avoidance of organic solvents,more kinds of identified components,non-destructive sampling and is therefore more suitable for the analysis of volatile components in chestnut rose juice.%采用顶空固相微萃取法（head-space solid phase micro-extraction,HS-SPME）和同时蒸馏萃取法（simultaneous distillation extraction,SDE）提取野生刺梨汁中挥发性成分,经气相色谱-质谱联用仪分析,结果共检测出52种挥发性化合物,其中烃类最多,有17种,其次是酯类12种和醇类7种,另外酸类、酚类、酮类、醛类和杂环类分别有5、3、3、3、2种。HS-SPME法和SDE法分别鉴定出37种和19种挥发性组分。两种提取方法相比较,HS-SPME具有快速简便、不使用溶剂、检测组分丰富和样品检测非破坏性等优点,更适宜于野生刺梨汁挥发性成分的分析。