构建食品级表达纳豆激酶的乳酸乳球菌重组菌株

摘要

构建整合型表达载体pFY008,以HisH基因作为同源重组的靶位点,通过同源重组将含有纳豆激酶原基因的表达盒PnisA-aprN整合到乳酸菌的基因组上,实现纳豆激酶在乳酸乳球菌中食品级的表达。构建的食品级基因工程菌安全性高、稳定性好,并赋予了其溶栓的功能。%An integrative expression vector designated as pFY008 was constructed with HisH gene as a homologous recombination target site.For food-grade expression of nattokinase gene(aprN) in Lactococcus lactis,the expression cassette PnisA-aprN gene was integrated into the genome of Lactococcus lactis by homologous recombination.A food-grade recombinant Lactococcus lactis was constructed to produce a thrombolytics protein.The recombinant Lactococcus lactis was found to have fibrinolytic activity.Meanwhile,it had fine stability and high safety.