新疆药桑椹中花色苷的分离与鉴定

摘要

This study dealt with the isolation of anthocyanins from Morus nigra Linn.fruits grown in Xinjiang by high-speed countercurrent chromatography(HSCCC).The solvent system used consisted of methyl tert-butyl ether,butanol,acetonitrile,water and trifluoroacetic acid(2:2:1:5:0.01,V/V).Fifty-gram sample was injected.As a result,4 individual anthocyanins with purities of 99.24%,88.5%,99.9% and 96%,respectively,were obtained.They were identified by HSCCC,UV-vis spectroscopy and LC-MS as cyanidin-3-O-rutinoside,pelargonidin-3-O-rutinoside,cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside,respectively.The HSCCC method was efficient,stable,simple and easy.%采用高速逆流色谱分离制备药桑花色苷。以甲基叔丁基醚-正丁醇-乙腈-水-三氟乙酸（2：2：1：5：0.01,V/V）为溶剂体系,进样量50mg,分离得到纯度分别为99.24%、88.5%、99.9%和96%的4个花色苷单体。通过高速逆流色谱（HSCCC）、紫外-可见光谱、质谱进行结构鉴定,初步确定馏分1为矢车菊-3-O-芸香糖苷,馏分2为天竺葵-3-O-芸香糖苷,馏分3为矢车菊-3-O-葡萄糖苷,馏分4为天竺葵-3-O-葡萄糖苷。此法高效、稳定、简捷易行。