为探讨乳酸钠对铜绿假单胞菌（Pseudomonas aeruginosa）的特定抑制作用，揭示乳酸钠质量浓度与介质pH值水平影响乳酸钠抑菌效果的规律，以营养肉汤培养基为介质，按照乳酸钠质量浓度（0、0．2、0．5、1．0、1．5、2．0g／100mL）单因素试验及乳酸质量浓度与介质pH值（4．0～6．2）的两因素交互试验设计，25℃恒温培养。结果表明：在适当的pH值范围内，乳酸钠（NaL）表现出良好的抑菌效果：当介质pH值小于NaL质量浓度的抑菌临界pH值时，NaL抑菌效果与其质量浓度成正比，介质pH4．8，抑菌效果2．0g／100mL NaL〉1．0g／100mL NaL〉0．5g/100mL NaL。NaL质量浓度一定，其抑菌效果与介质pH值成反比。%Nutrient broth media containing different concentrations of sodium lactate （0,0.2, 0.5, 1.0, 1.5 g/100 mE and 2.0 g/100 mL; consequent pH 4.0 -- 6.2） were used to culture Pseudomonas aeruginosa constantly at 25 ℃ to examine the inhibitory effect of sodium lactate on bacterial growth. In the proper pH range, sodium lactate showed outstanding antibacterial activity. At a medium pH level lower than the critical inhibitory pH, the antibacterial effect of sodium lactate was directly proportional to the corresponding concentration. At a medium pH of 4.8, sodium lactate exhibited the highest antibacterial activity at 2.0 g/100 mL, followed by 1.0 g/100 mL; the antibacterial activity was the weakest at 0.5 g/100 mL. At a constant sodium lactate concentration, the antibacterial activity was inversely proportional to medium pH.
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