大孔吸附树脂分离纯化蛹虫草固体培养基中虫草素工艺

摘要

比较D101、AB-8、HPD-100、HPD-400、HPD-500、HPD-722、DM130七种大孔吸附树脂对蛹虫草固体培养基中虫草素的吸附与解吸性能,筛选出HPD-100树脂为最佳树脂,并确定HPD-100树脂吸附分离最佳工艺条件：上样液质量浓度0.6mg/mL、上样流速3BV/h、上样体积6BV;解吸剂为体积分数25%乙醇溶液、解吸流速2BV/h、解吸体积4BV。根据此工艺条件,蛹虫草固体培养基粗提物经HPD-100树脂纯化后,虫草素产品纯度可达14.1%,较粗提物产品提高了8倍多。%The adsorption and desorption characteristics of macroporous resins D101,AB-8,HPD-100,HPD-400,HPD-500,HPD-722 and DM130 for cordycepin from spent solid culture medium after harvesting Cordyceps militaris fruit bodies were comparatively investigated.The results showed that HPD-100 was the best resin for the purification of cordycepin from the spent culture medium.The optimal separation and purification conditions were cordycepin concentration of 0.6 mg/mL,sample loading amount of 6 BV,sample loading flow rate of 3 BV/h,4 BV of 25% ethanol as desorbent,and desorption flow rate of 2 BV/h.Under these conditions,the purity of cordycepin could reach up to 14.1%,which was 8 times higher than before purification.