响应面试验优化辣木叶多酚超声辅助提取工艺及其抗氧化活性

摘要

为研究辣木叶多酚超声辅助提取工艺，明确辣木叶多酚体外抗氧化活性，选取超声功率、超声时间、超声温度和料液比为考察指标，研究不同工艺参数对辣木叶多酚提取量的影响，并采用响应面法优化辣木叶多酚提取工艺。此外，研究辣木叶多酚还原力及其对DPPH自由基、超氧阴离子自由基清除能力。结果表明，超声辅助提取辣木叶多酚最优工艺为：超声时间19.5 min、料液比1∶30（g/mL）、超声温度20.2℃、超声功率250 W。在此条件下，辣木叶多酚提取量为（25.14±0.46）mg/g。辣木叶多酚具有较强的体外抗氧化活性，其还原力、DPPH自由基和超氧阴离子自由基清除能力分别达到同等质量浓度VC的81.25%、94.15%和75.05%。该研究为辣木叶多酚等生物活性成分高效制备与抗氧化剂的深度开发提供理论依据。%In order to obtain the best ultrasound-assistedextracting conditions for polyphenols fromMoringa oleifera Lam. leaves, and clarified their antioxidant activities. Response surface methodology was used to explore the effects of ultrasonic power, extraction time, extraction temperature, and solid to liquid ratio on extraction rate. Moreover, the reducing power, the DPPH radical scavenging activities and the O2-· scavenging effect of polyphenols fromMoringa oleifera Lam. leaves were also investigated. The results showed that the best extraction condition were as follows: the extraction time of 19.5 min, the solid to liquid ratio of 1:30 (g/mL), the extraction temperature of 20.2℃, and the ultrasonic power of 250 W. Under the optimal extractions, the extraction rate of phycoerythrin was (25.14 ± 0.46) mg/g. In addition, the polyphenols fromMoringa oleifera Lam. leaves. have strong anti-oxidative, and the reducing power, the DPPH radical scavenging activities and the O2-· scavenging effect were 81.25%, 94.15% and 75.05% ofL-ascorbic acid, respectively. This research could provide the theoretical basis for higher preparation and antioxidants development ofMoringa oleifera Lam. leaves.