天然红色素灵菌红素的急性经口毒性和遗传毒性

摘要

Prodigiosin is a natural pigment that has the capability of inhibiting tumor cell proliferation in vitro,microbial growth,and exhibits anti-malaria and immunosuppression properties,thereby having a huge application potential.The application of prodigiosin in the food industry is limited due to the lack of knowledge about its acute oral toxicity and genetic toxicity.In the present study,according to the food safety national standards,the acute oral toxicity test of prodigiosin was performed in mice,and the genetic toxicity was assessed by mammalian erythrocyte micronucleus test,sperm malformation test and bacterial reverse mutation test.The results showed that acute oral medial lethal dose (LD50) of prodigiosin was greater than 10 g/kg BW.All three genetic toxicity tests gave negative results.In acute oral toxicity test,prodigiosin was found to be non-toxic to mice.Prodigiosin had no toxicity to normal cells and the reproductive system of animals.%灵菌红素属于天然色素,具有离体抑制肿瘤和癌症细胞增殖、抑制微生物生长以及抗疟疾和免疫抑制等功效,具有潜在的巨大应用价值.缺少急性经口毒性和遗传毒性实验的研究限制了灵菌红素在食品工业领域的应用.本研究依据食品安全国家标准进行了灵菌红素的小鼠急性经口毒性实验、哺乳动物红细胞微核实验、小鼠精子畸形实验和细菌回复突变实验.结果表明,灵菌红素急性经口半数致死剂量LD50＞10 g/kg(以体质量计),3项遗传毒性实验结果为阴性,灵菌红素对小鼠的急性经口毒性属无毒级,对正常细胞无毒,对动物生殖系统没有毒害.