大孔树脂固定化磷脂酶A1及其用于菜籽油脱胶工艺的优化

摘要

选择8 种大孔树脂对磷脂酶A1进行固定化,结果表明,离子交换树脂D001的固定化效果最好,其优化的最佳条件为缓冲液pH 5.0、酶添加量1.5 mL/g、固定化时间4 h,在该条件下获得的固定化酶活力为665.8 U/g.将固定化酶用于菜籽油脱胶实验,经响应面优化确定最优脱胶条件为固定化酶添加量1.8 g/kg、反应时间3.6 h、反应温度51 ℃、反应pH 5.5,在此条件下得到的脱胶油中磷含量为5.82 mg/kg.将固定化酶重复脱胶5 次后,仍保留初始酶活力的47.9%,脱胶油中磷含量为9.78 mg/kg.%Eight kinds of macroporous resins were comparatively used to immobilize phospholipase A1, and the best carrier was found to be D001. The optimum conditions for immobilization were as follows: pH 5.0, phospholipase dosage 1.5 mL/g and 4 h reaction, yielding an activity of immobilized enzyme as high as 665.8 U/g. The degumming of rapeseed oil with the immobilized enzyme was optimized using response surface methodology. The optimum conditions were determined as follows: immobilized enzyme dosage 1.8 g/kg, pH 5.5, reaction time 3.6 h, and reaction temperature 51 ℃. The phosphorus content of degummed oil prepared under these condition was 5.82 mg/kg. After fifth repeated use, the immobilized enzyme still retained 47.9% of its initial activity and the phosphorus content of degummed oil was 9.78 mg/kg.