红树莓多酚的组分分析及体外抗脂质过氧化活性

摘要

采用紫外-可见分光光度法、红外光谱法和高效液相色谱法,分析了'菲尔杜德'红树莓多酚粗提液和纯化液的组成成分,同时采用3 个体外抗脂质过氧化指标研究了其抗脂质过氧化活性.组分分析结果表明红树莓多酚中可能含有黄酮类、花色苷、糖苷类物质,初步鉴定出红树莓多酚中含有原花青素B2、芦丁、水杨酸和鞣花酸4 种酚类物质,且红树莓多酚粗提液和纯化液中多酚组成具有差异性.抗脂质过氧化活性结果表明:在一定质量浓度范围内,红树莓多酚粗提液和纯化液对Fe2+诱导卵黄脂蛋白脂质过氧化、H2O2诱导大鼠红细胞氧化溶血和大鼠肝组织匀浆自发脂质过氧化具有抑制作用,且呈现剂量依赖关系;红树莓多酚粗提液的抗脂质过氧化活性强于纯化液.红树莓多酚提取物对脂质过氧化有抑制作用,但其作用机制和途径仍需进一步研究.%Ultraviolet-visible spectroscopy, infrared spectroscopy and high performance liquid chromatography were applied to explore the compositions of crude and purified polyphenols from "Fertod Zamatos" red raspberry, and the anti-lipid peroxidation activity of red raspberry polyphenols was evaluated by three in vitro assays.The composition analysis showed the presence of flavonoids, anthocyanins and glycosides in red raspberry polyphenols. Proanthocyanidins B2, rutin, salicylic acid and ellagic acid were detected in red raspberry polyphenols, and there were some differences in the phenolic profile between the crude and purified polyphenols. Red raspberry polyphenols in a certain concentration range had inhibitory effects on Fe2+-induced lipid peroxidation in yolk lipoprotein, H2O2-induced hemolysis of rat red blood cells and spontaneous lipid peroxidation in rat liver tissue homogenate in a concentration-dependent manner. But crude red raspberry polyphenols showed stronger anti-lipid peroxidation activity than the purified one. Therefore, the polyphenol extracts from red raspberry have anti-lipid peroxidation activity; however, further studies are needed to investigate the underlying mechanism and pathways.