响应面分析优化加压溶剂法提取甜菊糖苷工艺

摘要

Response Surface Analysis was applied to optimize process conditions of stevioside extracted by pressurized Liquid method. Three factor, namely extraction pressure, extraction time and extraction temperature were selected. Base on single-factor experiments, stevioside extraction rate was as response value, and the effect of such factors on stevioside extraction rate was studied. The results showed that stevioside extraction rate can reach the highest level of 91.7 mg/g when follow this process conditions:material-liquid ratio was 1∶15 (g/mL), extraction pressure was 11 MPa, extraction time was 12 min and extraction temperature was 118 ℃. The experimental values agreed with those predicted values.%采用响应面分析优化加压溶剂法提取甜菊糖苷的工艺，在提取压力、提取时间、提取温度3个因素试验的基础上，以甜菊糖苷得率为响应值，考查因素对甜菊糖苷得率的影响。试验结果表明，在料液比为1∶15 g/mL，提取压力11 MPa、提取时间12 min和提取温度118℃时，甜菊糖苷得率达91.7 mg/g。试验结果与理论预测值基本相符。