草鱼鱼鳞胶原蛋白肽的制备及分析

摘要

Grass carp fish scale is byproduct of freshwater product. To obtain the collagen peptide, we hydrolyzed the Grass carp fish scale using Papain, and optimized the enzymatic hydrolysis conditions. Moreover, molecular weight of the collagen peptide was measured by Tricine-SDS-PAGE gel electrophoresis and Gel permeation chromatography. The results showed that the optimal hydrolysis conditions was temperature 60℃, enzyme dosage 8% (mass fraction), hydrolysis time 2 h, pH 5.0, material liquid ratio 1∶30. Molecular weight of the collagen peptide was less than 500 u, which mainly contains Glycine, Alanine, Hydroxyproline, Arginine and Aspartic acid.%草鱼鱼鳞为淡水产品副产物。我们用木瓜蛋白酶水解草鱼鱼鳞并优化酶解条件，运用Tricine-SDS-PAGE凝胶电泳和凝胶渗透色谱确定所得胶原蛋白肽的分子量，并分析了其中的氨基酸组成。结果表明，最佳酶解条件为：酶解温度60℃、酶添加量8%（质量分数）、酶解时间2 h、pH 5.0、料液比1∶30（g/mL）。所得的胶原蛋白肽分子量小于500 u，其中主要含有甘氨酸、丙氨酸、羟脯氨酸、精氨酸和天冬氨酸。