脂肪酶水解椰子油动力学研究

摘要

A kinetic study on hydrolysis for the production of derivatives catalyzed by Lipase was investigated. The effects of substrate concentration,enzyme concentration,temperature and time on the hydrolysis rate of co-conut oil by lipase were analyzed. Based on this,the Michaelis constant(km) and maximum rate(Vm) were de-duced by Lineweaver-Burk plotting and Wilkinson statistical method. Results showed that,under the optimal conditions of enzyme concentration 1%and temperature 50℃,the km and Vm were 1.273 9 [mg/(g·mL)] and 0.969 6 [mg/(g·mL·min)] respectively. The Michaelis-Menten equation was:v= 0.969 6[S]1.273 9+[S] . The model-pre-dicted values were consistent with the actually measured values from confirmation tests. Though the simulation of Michaelis-Menten equation to the hydrolysis process,goodness of fit were greater than 0.99,it found that ap-plication of Michaelis-Menten equation in hydrolysis process was reasonable. It can provide research foundation for the fat digestion process.%为了探究脂肪酶水解椰子油的动力学过程，在研究了底物质量浓度、酶添加量、酶解温度及酶解时间对脂肪酶水解椰子油反应速率影响的基础上，本试验采用Lineweaver-Burk法和Wilkinson统计法两种方法对酶解过程进行拟合，计算酶解过程的动力学常数km和Vm，并求解脂肪酶水解椰子油动力学方程。结果表明：在酶添加量为1%、温度为50℃的条件下，动力学常数km为1.2739[mg/（g·mL）]，Vm为0.9696[mg/（g·mL·min）]，米氏方程为：v=0.9696[S]1.2739+[S]。经过试验验证得出米氏方程的拟合度大于0.99，说明方程的预测值与测定值基本吻合，米氏方程适合脂肪酶水解椰子油动力学研究，为油脂酶解过程提供理论模型。