菜籽多肽微胶囊工艺优化及其抗氧化性研究

摘要

Rapeseed peptides possessed antioxidative,immune activity and other biological activities,but the low stability limits its application in functional food and other fields. The effect of the ratio of wall core, ulstrasonic temperature,time and power on embedding rate of rapeseed petides was studied.The results of Box-Behnken design show the ratio of wall core,ulstrasonic temperature and time had obvious effects on embedding rate. The optimal condition was determined by response surface test: the ratio of wall core was 20 : 1, ulstrasonic temperature was 45 ℃, ulstrasonic time was 76 min, and ulstrasonic power was 70 W. The embedding rate of rapeseed petides under this condition was 74.07 %.The oxygen radical absorbance capacity (ORAC)value of microcapsule obtained under this condition was 600 μmol TE/(g/sample)after 10 days.All results showed the optimal condition improved the stability of rapeseed peptides and the application.%菜籽多肽在抗氧化和增强免疫方面具有较强的功能活性,但稳定性较低,限制了其在食品加工领域的应用与发展.研究了壁芯比、超声温度、时间和功率对β-环糊精包埋菜籽多肽的影响;通过Box-Behnken实验设计发现壁芯比、超声时间和温度这3种因素对包埋率具有显著的影响.通过响应面试验确定β-环糊精包埋菜籽多肽的最佳工艺参数为:壁芯比为20:1,超声时间为76 min,温度为45℃,功率为70 W,此时的包埋率达到74.07 %.该条件下制备微胶囊置于空气中10天后抗氧化能力指数值大于600 μmol TE/(g/样品),说明该条件下制备微胶囊使得菜籽多肽的稳定性增强,具有一定的实际应用价值.