To get the yeast strains that can produce 4-hydroxy-2, 5-dimethyl-3(2H)-furanone(HDMF) using D-fructose as precursor of the fermentation, the yeasts were screened from soy sauce mash. 511 Yeast strains producing HDMF were separated by dilution method, in which 6 strains, Iiquid4-11, 17, 20, 27, 38, 39 had high ability to yield HDMF from20. 237 07 mg/L to 27. 893 25 mg/L. Strains of Iiquid4-11, 17, 27, 38, 39 were indentified as Candida versatilis, and Iiquid4-2O was Zygosaccharomyces pseudorouxii by 26S rDNA D1/D2 area sequential analysis and phylogenetic analysis.%以酱醪作为分离源,筛选可以利用D-果糖作为发酵前提物的,能产生4-羟基-2,5-二甲基-3[2H]-呋喃酮(HDMF)的高产酵母菌株.采用稀释法及多种培养基,共分离得到511株酵母菌,其中有6株高产菌株,为liquid 4-11、17、20、27、38、39,产量为20.24～27.90 mg/L.采用分子生物学鉴定方法,经26S rDNA D1/D2区序列分析及系统发育分析,初步将liquid 4-11、17、27、38、39鉴定为Candida versatilis,liquid 4 20鉴定为Zygosaccharom yces pseudorouxii.
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