以蓝莓果渣为原料,用酒石酸酸化乙醇提取花青素,同时应用超声波进行辅助提取,采用双波长pH示差法计算花青素提取率,利用响应面分析法对蓝莓果渣花青素的提取工艺条件进行优化.结果表明:其最佳提取条件为提取温度50℃,pH 3.0,酸化乙醇浓度65％,料液比1∶70(g/mL),超声波提取功率500 W,提取时间20 min,蓝莓果渣花青素提取率实际值为83.638％,与预测值85.442％的偏差为2.111％.该试验设计操作简便合理,得率高,可预测性较好,为蓝莓果渣花青素提取提供了理论依据.%The optimization of the ultrasonic extraction of anthocyanins in blueberry pomace by the response surface method was investigated in this study.With tartaric acid acidification ethanol extracting anthocyanins from blueberry pomace,with appling ultrasonic assisted extraction,anthocyanin extraction rate was determined by using the double wavelength pH differential method.Through the single factor experiment,to determine the best conditions were determined,and then the extraction process conditions of anthocyanins in blueberry pomace were optimized using the response surface analysis.The results showed that the extraction conditions on the influence of the anthocyanins blueberry pomace extraction yield order was as follows.i.e.material liquid ratio ＞ ethanol concentration ＞ extraction time ＞ ultrasonic power.Moreover,the best extraction condition was extracting it by using acidic ethanol concentration 65％ (pH 3.0),with material liquid than 1 ∶ 70,and 500 W ultrasonic,at 50 ℃ for 20 min.It was found that the anthocyanins in blueberry pomace extraction yield the actual value was 83.638％,only showing a 2.111 ％ deviation to the forecasting one (85.442 ％).In conclusion,the reasonable design of experiments was easy to handle,with high acquirement rate and good predictability,and this provided for the extraction of anthocyanins blueberry pomace a theoretical basis.
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