Total flavonoids in phellodendron was extracted by alcohol -ammonium sulfate two -phase aqueous system synergism with ultrasound extraction. Extraction time, ethanol-water ratio, material-solvent ratio and salt concentration on extraction ratio of total flavonoids were studied by experiment of single factor. Basing on single factor experiment, extraction time, ethanol-water ratio and material-solvent ratio were as response factors. Extraction ratio of total flavonoids from phellodendron was as response value. According to Box-Benhnken principle of design, the response surface method included three response factors and three levels and influence conditions on extraction ratio of total flavonoids were determined. The results of experiment showed that the maximum technical conditions were: extraction time 77min, material-solvent ratio l:26(m/V), ethanol-water ratio 0.71(V/V).%使用超声辅助乙醇-硫酸铵双水相体系提取黄柏中的总黄酮,通过单因素实验考察提取时间、醇水比、料液比和分相盐浓度对总黄酮提取率的影响.在此基础上以提取时间、醇水比和料液比为响应因素,黄柏中总黄酮的提取率为响应值,根据中心组合(Box-Benhnken)试验设计原理,采用三因素三水平的响应面分析法,确定各工艺条件对提取率的影响.结果表明超声辅助乙醇-硫酸铵双水相体系提取黄柏中的总黄酮的最佳工艺条件是:提取时间77min,料液比1∶26,醇水比是0.71.
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