A new protocol to analyze tetracyclines and sulfonamides from pig muscle was developed. Six antibiotics, oxytetracycline, tetracycline, chlortetracycline, slfamonomethoxine, sulfamethazine, and sulfamerazine, were analyzed by microbial assays and HPLC. Muscle juice samples were screened with Bacillus sterothermophilus in 96-well microplate. At the same time, the muscles samples were extracted with Mcllvaine-EDTA buffer solutions and then were detected at 270nm and 360nm. The detection limits of microbial assay were 0.02 ～ 0. 05μg/mL. For HPLC method, the limits of detection were 0.03 ～0.06 mg/kg, and the average recoveries were 80.25 ％ ～ 118.60％.%建立了一种采用高通量微生物法进行筛检,再用反相高效液相色谱法同时测定猪肉中土霉素、四环素、金霉素、磺胺甲基嘧啶、磺胺二甲嘧啶和磺胺间甲氧嘧啶的分析方法.样品取汁在96孔微板上采用嗜热脂肪芽孢杆菌(Bacillus sterothermophilus)进行筛检,同时采用Mcllvaine-EDTA溶液提取,选择270 nm和360 nm为检测波长进行检测.结果表明:所建立的微生法的检出限为0.02～0.05 μg/mL,HPLC法的检出限为0.03～0.06 mg/kg,添加回收率80.25 %～118.60%.
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