枣果中环磷酸腺苷（cAMP）的提取工艺及含量测定

摘要

大枣中的环磷酸腺苷（cAMP）含量丰富而且稳定，在已测的动植物材料中居首位。试验采用超声水浴提取的方法，考察超声时间、乙醇浓度、料液比3个因素对cAMP提取率的影响，结果表明料液比为主要影响因素，超声时间、乙醇浓度次之；确定了cAMP最佳提取：工艺：料液比1：25，乙醇浓度15％，超声时间20min。并采用高效液相色谱法测定了8种枣果中的cAMP的含量，最高的为朝阳圆枣（375．61μg／g），最低为1-早脆王（46．01μg／g）。%The cAMP content is abundant and stable in jujubes,and is the highest in animals and plants tested so far. This paper used the method of ultrasound and water bath extraction to study the effect of three factors （time of ul-trasound,concentration of alcohol,solid-liquid ratio） on the extraction rate of cAMP. The result showed that solid-liq-uid ratio was the main influencing factor, ultrasonic time, concentration of alcohol was then followed. The best extrac-tion process of cAMP was ： solid-liquid ratio 1：25, solvent coneentration15% ,ultrasonic time 20min. The paper also used high performance liquid chromatographic to determine cAMP content in eight kinds jujubes, and it showed that chaoyangyuanzao had the highest （ 375.61 μg/g content, 1 -zaocuiwang had the lowest （46.01μg/g） content.