Nanofiltration Extraction and Purification Method for Cyclic Adenosine Monophosphate (cAMP) from Chinese Date Fruit

摘要

Cyclic adenosine monophosphate (cAMP) is a kind of important bio-active substances. The cAMP content of Chinese date fruit is particularly abundant and stable, more than any of the other plants researched so far. In this chapter, we report the development of nanofiltration extraction method for cAMP from date fruit (jujube) paste. The paste was hydrolyzed at 50 °C with 2.5 g/L pectinases (pectin lyase, cellulose, and hemicellulose) for 3 h. The yield efficiency of date juice achieved 70 %, and most importantly, the extracted cAMP content increased from 10.8 to 15.7 mg/100 g. The results prove that pectic substances have a protective effect to ring adenosine phosphate After resin adsorption, elution with formic acid, and filtration through a 150-500 Da Nanofiltration membrane, the crude cAMP content was increased to 36 %. The purification percentage of cAMP using silica gel column chromatography was as much as 97 %. The date fruits were soaked and pureed, and then subjected to enzyme hydrolysis, filtration, resin adsorption, formic acid elution, and nanofiltration. Pure cAMP was obtained after purification via a silica gel chromatography column. The byproduct of cAMP extraction could be further developed, which might enable a nonpolluting industrial chain of products.