微波辅助水酶法优化红枣中环磷酸腺苷(cAMP)提取工艺及其抗疲劳作用

摘要

以新疆红枣为原料提取环磷酸腺苷(cyclic adenosine 3',5'-monophosphate,cAMP),通过响应面法优化微波辅助复配酶法提取cAMP工艺,并建立回归模型;同时研究cAMP提取物对D-半乳糖诱导衰老模型小鼠的游泳耐力的影响.结果表明,最佳提取工艺为复配酶(纤维素酶-半纤维素酶-果胶酶)质量比1∶1∶1、复配酶添加量1.5％(质量分数)、酶解温度31.3℃、酶解时间20 min、微波功率500 W,在此条件下cAMP提取量为560.9μg/g;新疆红枣cAMP提取物能延长小鼠力竭游泳时间,增加小鼠游泳耐力,说明cAMP对小鼠具有很好的抗疲劳作用.新疆红枣作为cAMP的提取原料在食品和医药工业中具有较好的应用前景.%Response surface methodology was used to optimize the microwave-assisted aqueous enzymatic extraction of cyclic adenosine 3',5'-monophosphate (cAMP) from Chinese jujube (Zizyphusjujuba Mill.) grown in Xinjiang.The antifatigue effect of the cAMP extracted was investigated.The optimal extraction conditions was found as follows:enzyme concentration 1.5％ (a mixture of cellulase,hemicellulase and pectinase,1∶1∶1 (m/m)),temperature 31.3 ℃,time 20 min and microwave power 500 W.Under these conditions,the yield of cAMP was 560.9 μg/g.The results showed that the cAMP could extend exhaustive swimming time and enhance swimming endurance in mice,suggesting good anti-fatigue effect.Therefore,cAMP holds great promise for its application in the food and pharmaceutical industry.