牦牛乳酪蛋白酶解物对H2O2诱导的氧化损伤HepG2细胞蛋白羰基含量及抗氧化酶活性的影响

摘要

为探究牦牛乳酪蛋白酶解物缓解氧化应激损伤的作用,利用过氧化氢(H2O2)建立氧化损伤细胞模型,以细胞蛋白质羰基含量及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)的活性为指标评价牦牛乳酪蛋白酶解物的活性.结果表明,利用酶解物处理H2O2损伤的HepG2细胞,能够缓解H2O2诱导的蛋白质羰基含量升高,并增加细胞内SOD、CAT、GSH-Px的活性,从而提高机体抗氧化能力,缓解H2O2引发的氧化应激状态.研究证明,牦牛乳酪蛋白酶解物具有缓解氧化应激损伤的作用,为牦牛乳资源的进一步开发利用及乳源活性肽应用于抗氧化功能食品提供了依据.%To evaluate the antioxidative activity of yak milk casein hydrolysates in vitro,HepG2 cells were treated with H2O2 to establish the oxidative model.The protein carbonyl content and the activity of SOD,GSH-Px and CAT of HepG2 cells pretreated with yak milk casein hydrolysates were measured to analyze the antioxidative activity of yak milk casein hydrolysates.The results showed that pretreatment with yak milk casein hydrolysates significantly alleviated the increase of protein carbonyl content induced by H2O2 and increased the activity of SOD,CAT and GSH-Px,suggesting the antioxidative capacity of yak milk casein hydrolysates in vitro.The results suggested that yak milk casein hydrolysates possessed protective effects against H2O2-induced oxidative damage in HepG2 ceils,which provided a basis for the further utilization of yak milk resources and for the application of the bioactive peptides as antioxidant functional foods.