云南省特色水果中多酚化合物含量和抗氧化性的比较

摘要

选用6种云南特色水果,利用Folin-Ciocalteau法测定可溶性和键合多酚含量,通过DPPH自由基清除能力、过氧化氢清除能力、总抗氧化能力、铁离子还原/抗氧化能力和还原能力测定其体外抗氧化活性,并对多酚含量和抗氧化活性进行比较.结果表明,香椿果中总多酚和键合多酚含量最高,分别达到(91.85±7.48)和(35.69±1.29)μmol FAE/g,与其他5种水果呈差异显著(p＜0.05),鸡嗉子果中可溶性多酚含量最高,达(60.28 ±7.98) μmol FAE/g,与其他水果呈差异显著(p＜0.05).香椿果中键合多酚的还原能力、总抗氧化能力和过氧化氢清除能力最高,多依果中可溶性多酚的总抗氧化能力和DPPH自由基清除能力最强,说明多酚化合物的抗氧化能力与水果品种、多酚含量和多酚种类存在一定关系.%The total phenolic contents and antioxidant activities of soluble polyphenol and insoluble bound poly phenol were determined in order to compare the differences among 6 kinds of selected fruits.The total phenolic content was determined by Folin-Ciocalteu method,the antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity,ferric reducing antioxidant power(FRAP),trolox equivalent antioxidant capacity (TEAC),reducing power (RP) and hydrogen peroxide (H2O2) scavenging activity in vitro.Results showed that total polyphenol and insoluble bound polyphenol contents of toona sinensis were the highest (respectively 91.85 ± 7.48 and 35.69 ± 1.29 μmol FAE/g);the two fractions were significantly higher than others (p ＜ 0.05).Soluble polyphenol contents were the highest in Dendrobenthamia capitata (60.28 ± 7.98 μmol FAE/g),and was significantly higher than others (p ＜ 0.05).The reducing power (RP),hydrogen peroxide (H2O2) scavenging activity and trolox equivalent antioxidant capacity (TEAC) of insoluble bound polyphenols in Toona sinensis were the highest,the trolox equivalent antioxidant capacity (TEAC) and DPPH radical scavenging activity of soluble polyphenols of Docynia delavayi were higher among 6 fruits.The results showed that the antioxidant capacity of polyphenol may be related to the variety of fruit,polyphenol content and phenolic compounds.