南疆传统发酵酸奶中可产生生物膜乳酸菌的筛选及鉴定

摘要

为了探索新疆长寿地区传统发酵酸奶中优势乳酸菌的生物膜形成能力,采用纯培养法、96孔微量板定量检测法、扫描电子显微镜观察法及16S rRNA基因序列分析法,对3份阿图什和l份乌什传统发酵酸奶中可产生生物膜的乳酸菌进行了筛选、分子鉴定及生物膜结构图片观察.最终从4份样品中分离出57株乳酸菌,其中38株(73.68％)乳酸菌均有不同程度的生物膜形成能力,16株(28.07％)乳酸菌为强黏附成生物膜菌株、22株(38.59％)为中等黏附成生物膜菌株.分子鉴定实验结果显示,可形成生物膜的乳酸菌共有3个属、6个种,其中16株为Enterococcus durans、1株为Enterococcusfaecium、7株为Enterococcus thailandicus、3株为Enterococcus lactis、6株为Lactobacillus plantarum、5株为Streptococcus thermophilus.可产生生物膜乳酸菌中肠球菌(Enterococcus)为优势菌属,其次为乳杆菌属(Lactobacillus)和链球菌属(Streptococcus).%To explore the Lactic acid bacteria diversity and biofilm formation in traditional fermented milk from Xinjiang longevity region,the samples were analyzed using pure culture and 16S rRNA gene sequence analysis,96 well micro-plate semi-quantitative methods and scanning electron microscopy.Finally 57 strains were isolated from four samples.The micro-plate semi-quantitative method was used to investigate biofilm formation of strains.The resuits showed that 38 strains (73.68％) of the LAB showed biofilm formation ability,of which 16 strains (28.07％) strongly adhered to biofilm and 22strains (38.59％) were moderately adherent.The strains which can form biofilm were belonging to three species of six genera including Enterococcus durans (16 strains),Enterococcusffaecium (1 strains),Enterococcusthailandicus (7 strains),Enterococcus lactis (3 strains),Lactobacillus plantarum (6 strains),Streptococcus thermophilius (5strains),in which Enterococcus was the dominant species and was followed by Lactobacillus and Streptococcus.These provided foundation for the development and utilization of LAB resources and their biofilm characteristics.