甜菊双糖苷/γ-环糊精包合物的制备

摘要

甜菊双糖苷(sbio)是一种典型的甜菊糖苷,用于蔗糖替代品,同时还具有多种药理活性.为了克服sbio 的水相溶解性差的缺点,以γ-环糊精(γ-CD)为主体分子,sbio为客体分子,包合形成高溶解度包合物.通过AutoDock4.2软件模拟确定sbio和γ-CD二者能够形成稳定的包合物;根据相溶解度曲线,确定sbio包合类型属于AL型,即包合比为1:1,并根据表观稳定常数Ks,包合过程热力学函数ΔG、ΔH和ΔS确定包合过程为吸热、熵增、自发过程;通过FTIR、SEM、XRD和1HNMR表征证实制备得到包合物.%Sbio is a typical steviol glycoside,and is used as sucrose substrate and possess many pharmaceutical values.In order to overcome Sbio poor aqueous solubility,Sbio/γ-CD was prepared by a co-evaporation method.Preliminary,the possible binding mode of Sbio with γ-CD was identified using AutoDock software.The phase solubility curve displayed a typical AL-type,which is 1∶1 inclusion complex.The inclusion is an endothermic,spontaneous and positive entropy process according to the apparent stability constant (Ks),inclusion thermos-dynamical functions,ΔG、ΔH and ΔS.Fourier transform infrared spectroscopy (FT-IR),Scanning electron microscope (SEM),X-ray diffraction (XRD) and NMR were tested to characterize the Sbio/γ-CD.