瘢痕疙瘩组织中胶原合成情况的研究

摘要

Objective To investigate the cause of excessive collagen accumulation in keloid tissue. Methods The ultrastructure of keloid was observed by transmission electron microscope. New formed collagen in keloid was localized with ABC immunohistochemical staining. Type I procollagen mRNA level in keloid tissue was determined by dot blot hybridization using human pro-al (I)collagenspecific cDNA probe. Results Numerous fibroblasts with abundant, well developed rough endoplasmic reticulum were exhibited in the ultrastructure of keloid. The type I procollagen mRNA levels were significantly increased in kreloid tissue. Immunohistochemical staining showed increased expression of new formed, type I procollagen in keloid tissue. Conclusion the fibroblasts are activated in collagen synthesis in active keloid. The enhanced collagen synthesis by fibroblasts is a critical factor leading to the overabundant collagen accumulation in keloid.%目的探讨瘢痕疙瘩中胶原过度积聚的形成原因。方法用透射电镜观察瘢痕疙瘩的超徽结构。用ABC法免疫组化检测瘢痕疙瘩中新合成的胶原。用地高辛标记的人I型前胶原al(I)cDNA探针与从瘢痕疙瘩和正常皮肤组织中提取RNA进行斑点杂交。结果在超徽结构上，多数瘢痕疙瘩成纤维细胞拥有丰富的高度发达的粗面内质网。斑点杂交结果显示瘢痕疙瘩组织中I型胶原mRNA水平升高。免疫组化染色显示瘢痕疙瘩中成纤维细胞有丰富的I型前胶原表达。结论在活跃增生的瘢痕疙瘩中，成纤维细胞胶原合成功能处于活化状态，胶原合成增加是瘢痕疙瘩中胶原过度积聚的重要原因。