目的：研究NK及NKT细胞在乙型肝炎相关慢加急性肝衰竭（HBV-ACLF）患者外周血中的变化。方法用流式细胞仪分别检测25例健康对照者（HC）、40例慢性乙型肝炎（CHB）患者、26例 HBV-ACLF患者外周血淋巴细胞中NK、NKT细胞的比例，经统计分析，比较各组间NK或NKT的差异，同时分析 HBV-ACLF组中的NK或NKT细胞与乙型肝炎标志物或肝功能间的相关性。结果 HC、CHB、HBV-ACLF组外周血的 NK细胞比例分别为（15．0±6．0）％、（11．4±6．8）％和（8．9±6．7）％，HBV-ACLF组与其他两组比较，差异有统计学意义（P＜0．05）；而NKT细胞在 HC、CHB、HBV-ACLF组外周血中的比例分别为（1．9±1．3）％、（4．3±3．7）％和（5．4±8．6）％，HBV-ACLF组与HC组比较，差异有统计学意义（P＜0．05）。结论 HBV-ACLF患者外周血内 NK细胞比例显著性降低，而 NKT 细胞比例显著性升高，他们可能在 HBV-ACLF疾病中发挥着一定的作用。%Objective To investigate the change in the proportion of NK or NKT cells in the peripheral blood of patients with HBV associated acute-on-chronic liver failure(HBV-ACLF) .Methods the frequency of NK or NKT cells in the blood of 25 healthy controls(HC) ,40 patients with chronic hepatitis B(CHB) and 26 HBV-ACLF patients was detected by flow cytometry .The differ-ences in the proportion of NK and NKT cells among the three groups were analyzed by SPSS software and the correlation was ana-lyzed between the frequence of NK or NKT cells and HBV markers and the level of liver function .Results the proportion of NK cells in HC ,CHB ,or HBV-ACLF group was (15 .0 ± 6 .0)% ,(11 .4 ± 6 .8)% ,(8 .9 ± 6 .7)% respectively ,and the difference be-tween the HBV-ACLF group and HC or CHB group was statistically significant (P<0 .05) .And for the NKT cells ,its frequence in the HC ,CHB ,or HBV-ACLF group was (1 .9 ± 1 .3)% ,(4 .3 ± 3 .7)% ,(5 .4 ± 8 .6)% respectively ,and there was significant difference between the HBV-ACLF group and HC group(P<0 .05) .Conclusion The proportion of NK cells in HBV-ACLF has a significant decline ,while NKT cells has a significant increases .it indicate that NK or NKT cells might be play a certain role in the HBV-ACLF development process .
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