恶臭假单胞菌发酵条件的研究

摘要

从未发病的K326烟株根际土壤中筛选出对TMV具有高度拮抗活性菌株A3，发酵液的抑制效果可达95%以上，为此，从碳源、氮源、pH、温度、通气量及发酵时间等方面对该菌株的摇瓶发酵条件进行研究。结果表明，A3菌株发酵适宜温度为28℃，在该温度下发酵60～70 h达到菌体最大数量，且抑菌效价最高；以葡萄糖为适宜的碳源可以更好的促进菌量的生长和抗病毒物质的分泌，有机氮中酵母膏或蛋白胨对菌株的生长和抗病毒活性成分生成均能达到理想的效果，鱼粉则不能被A3菌株很好的利用；pH 7是菌株最适宜的发酵范围，偏酸不利于菌株的生长；适当加大通气量能更好地促进A3菌株的繁殖及抗病毒物质的分泌。%The bacterial strain A3 with high inhibition to TMV was isolated from healthy tobacco (K326) field. Its culture medium and fermentation conditions were investigated. The results showed that 28 oC was the most optimal fermentative temperature for A3 to grow and produce antiviral, and at optimal cultural temperature the amount and antiviral of A3 reached the highest level after 60-70 h respectively. Glucose was suitable carbon-source nutrition for the strain growth, and peptone or yeast was a suitable nitrogen source nutrition as well. The pH 7 was fit for A3 fermentation, and acidic condition was not favorable to the growth of strain A3. Ample air supplied markedly could promote growth and antiviral production of the strain A3.