恶臭假单胞菌产精氨酸脱亚胺酶发酵条件及特性的研究

摘要

对恶臭假单胞菌N0705产精氨酸脱亚胺酶的发酵条件及部分酶学性质进行了研究.结果表明,在培养温度28℃,培养基初始pH值为6.6,接种量4%,装液量40mL/250mL,发酵时间50h时,精氨酸脱亚胺酶酶活最大.精氨酸脱亚胺酶发酵粗酶液在温度37℃及pH 6.0～7.0时有较好的稳定性.%The fermentation conditions and the some properties of arginine deiminase (ADI) produced by Pseudomonas putida UN0705 were studied. The optimal fermentation conditions were as follows: liquid volume 40ml/250ml, initial pH value 6.6, inoculum 4%, culture temperature 28℃ and culture time Soh. Under these conditions, the high activity of arginine deiminase was obtained. The crude arginine deiminase was highly stable at 37℃ and in the pH range from 6.0 to 7.0.