培菲康对硫代乙酰胺诱导的大鼠肝性脑病的保护作用

摘要

AIM　The effect of bifico on experimental hepatic encephalopathy (HE) induced by thioacetamide(TAA) and the possible mechanism of its protective effect were studied. METHODS　Experimental hepatic encephalopathy was induced by ig thioacetamide 250 mg*kg-1 successively for two days; bifico was administraed ig once per day for one week before HE induction. RESULTS　Compared with control HE rat, Bifico improved rat neuro-reflexes score and hepatic injury grade(P<0.05); thus decreased rat serum ammonium and LPS concentrations, respectively (P<0.05,P<0.01). The preparation slightly increased the ratio of chain amino acid to aromatic amino acid as well as reduced the degree of liver necrosis. CONCLUSION　At the dosage of 840 mg*kg-1 ig for one week before liver intoxication, Bifico significantly protects rats from hepatic encephalopathy induced by TAA.%目的探讨培菲康对硫代乙酰胺（TAA）引起的大鼠肝性脑病（HE）模型的作用及其机制。方法利用大鼠TAA HE模型，观察活菌制剂培菲康对HE大鼠神经反射，血清氨浓度，血清氨基酸支/芳比值，肝门静脉LPS浓度和肝脏病理损伤的影响。结果培菲康能降低大鼠的HE等级，改善HE大鼠的神经反射；显著降低血氨和门静脉LPS浓度，升高氨基酸的支/芳比值，减轻肝脏的病理损伤。结论培菲康对TAA引起的大鼠HE有明显的保护作用，其作用机制与降低血氨、门静脉LPS浓度和升高血清氨基酸支/芳比值有关。