生大黄联合培菲康对重症急性胰腺炎大鼠的早期保护作用

摘要

Objective:To discuss the effect of the rhubarb combined with bifid tripleviable on the TNF-α,the IL-10 and the pancreatic histopathological changes in rats with severe acute pancreatitis. Methods:64 SD rats were divided randomly into the normal control group,the model group,the rhubarb group and the combination group. Rats models were established by the abdominal injection with 10% sinistral arginine solution. Concentrations including the TNF-α and the IL-10 were detected by the radioimmunoassay method. The ratio of wet and dry weight and the pancreatitis pathology integration were evaluation. Results:The TNF-a and the IL-10 of the model group were higher obviously than those of the normal control group. In the rhubarb group and the combination group, these indices were lower than those in the model group. Meanwhile, the ration of wet and dry weight and the pathology integration were also lower than those in the model group. The IL-10 in serum of the combination group was higher than it in the rhubarb group significantly. Conclusion:The balance of the pro-inflammatory cytokinces and the intestinal function can be improved by the rhubarb combined with the bifid tripleviable. Moreover, this combination can relieve the inflammatory reaction of the SAP and early protection of it.%目的 探讨生大黄和培菲康对重症急性胰腺炎(SAP)大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)和胰腺组织病理学改变的影响.方法 64只SD大鼠随机分正常对照组、模型组、生大黄组和联合组,用10％左旋精氨酸溶液腹腔内注射制作SAP大鼠模型.采用放射免疫法检测大鼠血清TNF-α、IL-10质量浓度,观察胰腺组织湿/干质量比,光镜下评估胰腺组织病理学积分.结果 模型组大鼠血清TNF-α、IL-10水平明显高于正常对照组；生大黄组和联合组大鼠血清TNF-α、IL-10水平低于模型组,其中胰腺湿/干质量比及组织病理评分均显著低于模型组；联合组大鼠血清IL-10水平高于生大黄组,差异具有统计学意义.结论 生大黄联合微生态制剂可导致SAP大鼠重建促抗炎细胞因子的平衡,促进肠道功能早期恢复,进而减轻SAP的炎症反应,对SAP起到早期保护作用.