Aim To investigate the effects of osthole ( Ost) on the ability of proliferation and differentiation in APP transduced neural stem cells( NSCs) , and neu-ronal apoptosis, in order to find related mechanism. Methods A model of Alzheimer′s disease( AD) cells was successfully established by transducing APP gene into NSCs in vitro. The ability of proliferation and dif-ferentiation was tested by staining. The viability of NSCs was determined by using CCK-8 assay. The cell apoptosis was tested by Hoechst 33258 staining. The expression of GSK-3β and β-catenin mRNA was deter-mined by RT-PCR. The expression of GSK-3β and β-catenin protein was determined by Western blot. Re-sults The ability of proliferation had increased by 10 . 24% with Ost treatment, compared with APP group. The ability of differentiation had increased by 6 . 74%with Ost treatment, compared with APP group. The vi-ability of NSCs had increased and cell apoptotic rate had decreased significantly. From the results of RT-PCR and Western blot, we could find the expression of GSK-3βmRNA and protein had decreased, and the ex-pression of β-catenin mRNA and protein had increased significantly, compared with APP group. Conclusion Ost could enhance the ability of proliferation and dif-ferentiation into more neurons of NSCs transducing APP gene, and reduce neuronal apoptosis. It might be relat-ed with activiting Wnt/β-catenin signaling pathway.%目的：通过转染APP基因于神经干细胞，研究蛇床子素对其增殖和分化能力的影响，及对神经元凋亡的影响，并研究其机制。方法体外建立阿尔茨海默病的神经干细胞模型，利用免疫组化染色法，研究蛇床子素对转染APP基因的神经干细胞增殖和分化能力的影响；通过CCK-8法检测神经干细胞存活率；Hoechst 33258法检测神经元凋亡情况；RT-PCR法检测 GSK-3β和β-catenin mRNA 的表达变化情况；Western blot法检测 GSK-3β和β-catenin 蛋白的表达变化情况。结果与APP组相比，蛇床子素组的神经干细胞增殖能力提高了10．24%；分化为神经元的能力提高了6．74%。与APP组相比，蛇床子素组神经干细胞的存活率明显提高；神经元凋亡明显减少；RT-PCR和Western blot结果显示，蛇床子素可抑制GSK-3β基因和蛋白的表达，促进β-catenin基因和蛋白的表达。结论蛇床子素可促进转染APP基因的神经干细胞的增殖，可促进其分化为更多的神经元，并减少神经元凋亡，可能与通过激活Wnt/β-catenin信号通路有关系。
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